Biomimetic Materials and Tissue Engineering Laboratory, Department of Chemical Engineering, University of South Carolina, Columbia, SC 29208, USA.
Curr Opin Biotechnol. 2011 Oct;22(5):655-60. doi: 10.1016/j.copbio.2011.01.003. Epub 2011 Feb 8.
Success of tissue engineered constructs in regenerative medicine is limited by the lack of cellmatrix interactions to guide devleopment of the seeded cells into the desired tissue. This review highlights the most exciting developments in bioconjugation of synthetic hydrogels targeted to tissue engineering. Application of conjugation techniques has resulted in the synthesis of novel biomimetic cell-responsive hydrogels to control the cascade of cell migration, adhesion, survival, differentiation, and maturation to the desired lineage concurrent with matrix remodeling. The future outlook includes developing conjugated patterned hydrogel matrices, developing novel hydrogel matrices to support self-renewal and pluripotency of embryonic and adult stem cells, and merging 3D printing with bioconjugation to fabricate hydrogels with anatomical arrangement of cells and biomolecules.
组织工程构建体在再生医学中的成功受到缺乏细胞-基质相互作用的限制,这种相互作用无法指导种子细胞向所需组织的发育。本综述重点介绍了针对组织工程的合成水凝胶的生物共轭的最令人兴奋的进展。共轭技术的应用导致了新型仿生细胞响应水凝胶的合成,以控制细胞迁移、黏附、存活、分化和成熟到所需谱系的级联反应,同时进行基质重塑。未来的展望包括开发共轭图案化水凝胶基质、开发新的水凝胶基质以支持胚胎和成人干细胞的自我更新和多能性,以及将 3D 打印与生物共轭相结合,以制造具有细胞和生物分子解剖排列的水凝胶。