Department of Medical Microbiology and Immunology, University of Turku, Turku, Finland.
Eur J Immunol. 2011 Mar;41(3):634-44. doi: 10.1002/eji.201040760. Epub 2011 Feb 10.
Ly6C is a murine cell-surface antigen expressed by plasma cells, subsets of myeloid cells and many T cells, including memory T cells. We previously documented that Ly6C crosslinking induces LFA-1 clustering on naïve CD8(+) T cells. Here, we show that in vitro and in vivo differentiation of naïve CD8(+) T cells into central (Tcm) but not effector (Tem) memory T cells enhances Ly6C expression, and its crosslinking induces strong LFA-1 clustering on Tcm. Blocking Ly6C function inhibits in vivo Tcm homing to LNs as efficiently as blocking L-selectin but it does not potentiate the inhibition provided by blocking either L-selectin or LFA-1 function. Thus, Ly6C, L-selectin and LFA-1 all appear to be part of a common homing pathway. In vitro, Ly6C crosslinking enhances Tcm adherence to ICAM-1 in the presence of CCL21. In summary, Tcm homing involves Ly6C, in addition to L-selectin and LFA-1, and appears to potentiate firm adhesion of Tcm to ICAM-1 in synergy with a chemokine. We propose that Ly6C augments Tcm compartmentalization into LNs during their homing.
Ly6C 是一种在浆细胞、髓系细胞亚群和许多 T 细胞（包括记忆 T 细胞）表面表达的鼠源细胞表面抗原。我们之前的研究表明，Ly6C 交联诱导幼稚 CD8(+) T 细胞上的 LFA-1 聚集。在这里，我们发现，在体外和体内，幼稚 CD8(+) T 细胞分化为中央记忆 T 细胞（Tcm）而不是效应记忆 T 细胞（Tem），会增强 Ly6C 的表达，其交联会诱导 Tcm 上强烈的 LFA-1 聚集。阻断 Ly6C 功能可有效抑制 Tcm 向 LNs 的归巢，与阻断 L-选择素的效果一样，但不能增强阻断 L-选择素或 LFA-1 功能提供的抑制作用。因此，Ly6C、L-选择素和 LFA-1 似乎都属于一个共同的归巢途径。在体外，Ly6C 交联在 CCL21 存在的情况下增强 Tcm 对 ICAM-1 的黏附。总之，Tcm 归巢除了涉及 L-选择素和 LFA-1 之外，还涉及 Ly6C，并且似乎与趋化因子协同增强 Tcm 与 ICAM-1 的牢固黏附。我们提出，Ly6C 在 Tcm 归巢过程中增强了其向 LNs 的分区。
