Zhou Ting, Qian Tianxiu, Wang Xiaoying, Li Xianen, Cao Li, Gui Shuangying
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 151 Malianwa North Road, Haidian District, Beijing, 100193, People's Republic of China.
Biomed Chromatogr. 2011 Dec;25(12):1360-3. doi: 10.1002/bmc.1609. Epub 2011 Feb 10.
A highly sensitive, specific and simple LC-MS/MS method was developed to investigate in vivo bio-transformation of oleuropein in rat. Rat urine samples collected after the intravenous administrations were determined using liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the negative-ion mode. The assay procedure involves a simple liquid-liquid extraction of parent oleuropein and the metabolite from rat urine with ethyl acetate. Chromatographic separation was operated with 0.1% formic acid aqueous and methanol in gradient program at a flow rate of 0.80 mL/min on an RP-C(18) column with a total run time of 30 min. This method has been successfully applied to simultaneous determination of oleuropein and its metabolite in rat urine. Oxygenation was found to be the major metabolic pathway of the oleuropein in rat after intravenous administration.
建立了一种高灵敏度、高特异性且简单的液相色谱-串联质谱（LC-MS/MS）方法，用于研究橄榄苦苷在大鼠体内的生物转化。静脉给药后收集的大鼠尿液样本，采用负离子模式电喷雾电离的液相色谱-串联质谱法进行测定。该测定方法包括用乙酸乙酯从大鼠尿液中对母体橄榄苦苷及其代谢物进行简单的液-液萃取。在RP-C(18)柱上，以0.1%甲酸水溶液和甲醇为流动相，采用梯度洗脱程序，流速为0.80 mL/min，总运行时间为30 min进行色谱分离。该方法已成功应用于同时测定大鼠尿液中橄榄苦苷及其代谢物。结果发现，静脉给药后，氧化是大鼠体内橄榄苦苷的主要代谢途径。
