Wu G, Ju L, Jin T, Chen L, Shao L, Wang Y, Liu B
School of Stomatology, The Fourth Military Medical University, Xi'an, China.
J Int Med Res. 2010 Sep-Oct;38(5):1682-8. doi: 10.1177/147323001003800513.
This study explored the function and possible mechanism of bone morphogenetic protein-2 (BMP-2) in the healing of injured peripheral nerves in vivo. Rabbit facial nerves were injured by clamping and then treated with recombinant human BMP-2 (rhBMP-2) or phosphate-buffered saline (control) by injecting once during surgery and twice a day post-injury for 7 days. Facial nerve fragments within 5 mm of the clamping point were examined at different times post-surgery. Axon structures visualized by Bielschowsky staining were similar in experimental and control nerves 2 and 6 weeks post-injury. At 4 weeks post-injury, cross-section images of facial nerves showed that axons treated with rhBMP-2 were denser and thicker, and levels of tau protein were increased. It is concluded from these data that rhBMP-2 may affect injured facial nerve regeneration by inducing more neurons to return to embryonic patterns of tau gene expression.
本研究在体内探讨了骨形态发生蛋白-2（BMP-2）在周围神经损伤愈合中的作用及可能机制。通过夹闭法损伤兔面神经，然后在手术期间注射一次重组人BMP-2（rhBMP-2）或磷酸盐缓冲盐水（对照组），损伤后每天注射两次，持续7天。在术后不同时间检查夹闭点5mm内的面神经片段。损伤后2周和6周，经 Bielschowsky 染色显示的轴突结构在实验组和对照组神经中相似。损伤后4周，面神经横断面图像显示，经rhBMP-2处理的轴突更密集、更粗，且tau蛋白水平升高。从这些数据得出结论，rhBMP-2可能通过诱导更多神经元恢复tau基因表达的胚胎模式来影响损伤的面神经再生。
