Wang Yan-Liang, Wang Da-Zhang, Nie Xin, Lei De-Lin, Liu Yan-Pu, Zhang Yong-Jie, Suwa Fumihiko, Tamada Yoshitaka, Fang Yi Ru, Jin Yan
Department of Oral Maxillofacial Surgery, Hua Xi Stomatology Hospital, Sichuan University, Cheng Du 610041, P.R. China.
Br J Oral Maxillofac Surg. 2007 Apr;45(3):197-202. doi: 10.1016/j.bjoms.2006.06.003. Epub 2006 Jul 28.
We investigated the effects of bone morphogenetic protein-2 (BMP-2) and some other BMPs on regeneration of peripheral motor nerves in vivo. The facial nerves of 24 New Zealand rabbits were crushed to examine a series of retrograde changes in the facial nuclei and axons, in what has been called the "axon reaction". The facial nerves of the experimental group were treated with epineurial coaptation and BMP-2 after the injury. Nerves not treated with BMP-2 were regarded as controls. The expression of BMP-2 was investigated by in situ hybridisation in the neurons of facial nuclei. The electrophysiology, image analysis and transmission electron microscopy were used to evaluate the level of the recovery of facial nerves. The results showed that the axons in the experimental group were thicker and denser than those in the control group four weeks later. The expression of BMP-2 in the neurons of facial nuclei increased after injury. The electron microscopic observations showed that the axons' degeneration in the experimental group was less than that in the control group. Despite the morphological difference between the two groups, there was no apparent difference between them in nerve conduction velocity. These findings suggest that BMP-2 might be involved in the regeneration of facial nerves, and might function as a potential neurotrophic factor.
我们研究了骨形态发生蛋白-2（BMP-2）及其他一些骨形态发生蛋白对体内周围运动神经再生的影响。将24只新西兰兔的面神经切断，以观察面神经核和轴突中一系列逆行性变化，即所谓的“轴突反应”。实验组的面神经在损伤后采用神经外膜吻合术并使用BMP-2进行处理。未用BMP-2处理的神经作为对照。通过原位杂交技术研究面神经核神经元中BMP-2的表达情况。采用电生理学、图像分析和透射电子显微镜技术评估面神经的恢复程度。结果显示，四周后实验组的轴突比对照组的更粗且更密集。面神经核神经元中BMP-2的表达在损伤后增加。电子显微镜观察表明，实验组轴突的退变程度小于对照组。尽管两组在形态上存在差异，但它们在神经传导速度方面没有明显差异。这些研究结果表明，BMP-2可能参与面神经的再生，并可能作为一种潜在的神经营养因子发挥作用。
