Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, Virginia 20110, United States.
J Proteome Res. 2011 Apr 1;10(4):1944-52. doi: 10.1021/pr101179t. Epub 2011 Feb 28.
In this present work, we characterized the proteomes of pancreatic ductal adenocarcinoma (PDAC) cells and normal pancreatic duct cells by mass spectrometry using LTQ-Orbitrap, and identified more than 1200 proteins from each sample. On the basis of spectra count label-free quantification approach, we identified a large number of differentially expressed metabolic enzymes and proteins involved in cytoskeleton, cell adhesion, transport, transcription, translation, and cell proliferation as well. The data demonstrated that metabolic pathways were altered in PDAC, consistent with Warburg effect. Our analysis provides a potentially comprehensive picture of metabolism in PDAC, which may serve as the basis of new diagnostic and treatment of PDAC.
在本研究中，我们采用 LTQ-Orbitrap 质谱技术对胰腺导管腺癌（PDAC）细胞和正常胰腺导管细胞的蛋白质组进行了分析，从每个样本中鉴定出了 1200 多种蛋白质。基于谱图计数无标记定量方法，我们鉴定出了大量差异表达的代谢酶和参与细胞骨架、细胞黏附、运输、转录、翻译和细胞增殖的蛋白质。这些数据表明，PDAC 中的代谢途径发生了改变，与瓦博格效应一致。我们的分析为 PDAC 中的代谢提供了一个潜在的全面图景，可能为 PDAC 的新诊断和治疗提供依据。
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