Ming Dong Sheng, Heathcote John, Garg Arun, Darbyshire Jim, Eagleston Erna, Bajkov Teri-Lynn
Department of Chemistry, BC Biomedical Laboratories, 7455-130th Street, Surrey, British Columbia, Canada.
Bioanalysis. 2011 Feb;3(3):301-12. doi: 10.4155/bio.10.209.
To develop an UPLC-MS/MS method to replace the in-house immunoassay for the analysis of urinary cortisol.
Cortisol was extracted from human urine by ethyl acetate and analyzed on a Waters ACQUITY TQD system using a BEH C18 column. Linear calibration curves were generated over the range of 27.6 to 1380 nmol/l and exhibited consistent linearity and reproducibility with a correlation coefficient greater than 0.9950. Intra-day coefficients of variation were between 3.74 and 5.10% and inter-day coefficients of variations were between 4.22 and 6.73%. The extraction recovery of cortisol was greater than 83%.
An accurate, rapid and robust UPLC-MS/MS method for the determination of urinary cortisol has been developed and validated. With a lower flow rate (0.4 ml/min), a shorter running time per sample and a simple and cost-effective sample preparation, this method is a desirable option for clinical laboratories.
开发一种超高效液相色谱-串联质谱法（UPLC-MS/MS）以取代内部免疫分析法用于尿皮质醇分析。
用乙酸乙酯从人尿中提取皮质醇，并在沃特世ACQUITY TQD系统上使用BEH C18色谱柱进行分析。在27.6至1380 nmol/l范围内生成线性校准曲线，相关系数大于0.9950，显示出一致的线性和重现性。日内变异系数在3.74%至5.10%之间，日间变异系数在4.22%至6.73%之间。皮质醇的提取回收率大于83%。
已开发并验证了一种准确、快速且稳健的用于测定尿皮质醇的UPLC-MS/MS方法。该方法流速较低（0.4 ml/min），每个样品运行时间较短，样品制备简单且成本效益高，是临床实验室的理想选择。
