Hou Y M, Zhang Q J, Wang S J
Institute of Biophysic, Chinese Academy of Sciences, Beijing.
Chin J Biotechnol. 1990;6(4):269-77.
Gene coding for E. coli D-xylose isomerase was cloned into the plasmid pWR13 from the plasmid pXO100 containing D-xylose operon. Several subclones harboring DNA fragments of different size obtained by step- by-step shortening with Bal31 nuclease were directly sequenced. The complete nucleotide sequence of the 1.6kb DNA fragment carrying D-xylose isomerase gene was determined. The structural gene of 1320 nucleotides codes for a protein of 440 amino acids. The additional 209 nucleotides at 5' end and 82 nucleotides at 3' end include Shine-Dalgarno ribosome-binding site and the transcriptional termination codon, respectively.
将编码大肠杆菌D-木糖异构酶的基因从含有D-木糖操纵子的质粒pXO100克隆到质粒pWR13中。通过用Bal31核酸酶逐步缩短获得的几个含有不同大小DNA片段的亚克隆被直接测序。确定了携带D-木糖异构酶基因的1.6kb DNA片段的完整核苷酸序列。1320个核苷酸的结构基因编码一个440个氨基酸的蛋白质。5'端额外的209个核苷酸和3'端的82个核苷酸分别包括Shine-Dalgarno核糖体结合位点和转录终止密码子。
