Wilhelm M, Hollenberg C P
Nucleic Acids Res. 1985 Aug 12;13(15):5717-22. doi: 10.1093/nar/13.15.5717.
The xylose isomerase gene from Bacillus subtilis was cloned from a genomic BamH1 library by complementation of an isomerase defective Escherichia coli strain as previously described. The ATG initiation codon is preceded by a Shine-Dalgarno sequence and two hexamers being characteristic for the promoter region of Bacillus genes. The structural gene consists of 1320 base pairs, thus coding for a polypeptide chain of 440 amino acids with a molecular weight of 49 680. The polypeptide primary structure shows over 50% homology to that of the E. coli xylose isomerase.
如前所述，通过对一株异构酶缺陷型大肠杆菌菌株进行互补，从枯草芽孢杆菌基因组BamH1文库中克隆出了木糖异构酶基因。ATG起始密码子之前有一个Shine-Dalgarno序列和两个六聚体，它们是芽孢杆菌基因启动子区域的特征。结构基因由1320个碱基对组成，因此编码一条由440个氨基酸组成的多肽链，分子量为49680。该多肽一级结构与大肠杆菌木糖异构酶的一级结构具有超过50%的同源性。
