Keelan M, Wierzbicki A, Clandinin M T, Walker K, Thomson A B
Department of Medicine, University of Alberta, Edmonton, Canada.
Diabetes Res. 1990 Aug;14(4):165-70.
Female Wistar rats were fed for 8 weeks a high polyunsaturated fatty acid diet (P) enriched with 18:2w6 and 18:3w3, or a high saturated fatty acid diet (S) enriched with 16:0, 18:0 and 18:1w9. There was a similar jejunal BBM fatty acid profile in phosphatidylcholine (PC) and phosphatidylethanolamine (PE) composition in animals fed P. However, feeding S altered the fatty acid composition of BBM phospholipids: for PC, feeding S increased total monounsaturated fatty acids (e.g. 18:1(9)) and reduced total polyunsaturated fatty acids, especially the total w6 fatty acids. For PE, feeding S increased only the total monounsaturated fatty acids. The ratio of monounsaturated/saturated fatty acids was increased for PC and PE. Fatty acid changes were more pronounced in the jejunal than ileal BBM. It is concluded that alterations in dietary fatty acid saturation: (a) influences intestinal BBM phospholipid fatty acid composition; (b) these changes are quantitatively and qualitatively different for PC and PE; and (c) changes are greater in the proximal than in the distal intestine. It is proposed that the previously reported diet-associated changes in active and passive intestinal transport are due at least in part to these alterations in the fatty acid composition in brush border membrane phospholipids.
将雌性Wistar大鼠分别喂食富含18:2w6和18:3w3的高多不饱和脂肪酸饮食（P）或富含16:0、18:0和18:1w9的高饱和脂肪酸饮食（S），持续8周。喂食P的动物空肠刷状缘膜（BBM）中磷脂酰胆碱（PC）和磷脂酰乙醇胺（PE）的脂肪酸组成具有相似性。然而，喂食S改变了BBM磷脂的脂肪酸组成：对于PC，喂食S增加了总单不饱和脂肪酸（如18:1(9)）并减少了总多不饱和脂肪酸，尤其是总w6脂肪酸。对于PE，喂食S仅增加了总单不饱和脂肪酸。PC和PE的单不饱和/饱和脂肪酸比值均升高。空肠BBM中的脂肪酸变化比回肠BBM更明显。研究得出结论：饮食脂肪酸饱和度的改变：（a）影响肠道BBM磷脂脂肪酸组成；（b）PC和PE的这些变化在数量和质量上有所不同；（c）近端肠道的变化大于远端肠道。有人提出，先前报道的与饮食相关的主动和被动肠道转运变化至少部分归因于刷状缘膜磷脂脂肪酸组成的这些改变。
