Immunorheumatology and Tissue Regeneration Laboratory/RAMSES, Rizzoli Orthopaedic Institute, Bologna, Italy.
J Cell Biochem. 2011 May;112(5):1418-30. doi: 10.1002/jcb.23058.
With the continuous discovery of new alternative sources containing mesenchymal stem cells (MSCs), regenerative medicine therapies may find tailored applications in the clinics. Although these cells have been demonstrated to express specific mesenchymal markers and are able to differentiate into mesenchymal lineages in ad hoc culture conditions, it is still critical to determine the yield and differentiation potential of these cells in comparative studies under the same standardized culture environment. Moreover, the opportunity to use MSCs from bone marrow (BM) of multiorgan donors for cell banking is of relevant importance. In the attempt to establish the relative potential of alternative MSCs sources, we analyzed and compared the yield and differentiation potential of human MSCs from adipose and BM tissues of cadaveric origins, and from fetal annexes (placenta and umbilical cord) after delivery using standardized isolation and culture protocols. BM contained a significantly higher amount of mononuclear cells (MNCs) compared to the other tissue sources. Nonetheless, a higher cell seeding density was needed for these cells to successfully isolate MSCs. The MNCs populations were highly heterogeneous and expressed variable MSCs markers with a large variation from donor to donor. After MSCs selection through tissue culture plastic adhesion, cells displayed a comparable proliferation capacity with distinct colony morphologies and were positive for a pool of typical MSCs markers. In vitro differentiation assays showed a higher osteogenic differentiation capacity of adipose tissue and BM MSCs, and a higher chondrogenic differentiation capacity of BM MSCs.
随着不断发现新的含有间充质干细胞 (MSCs) 的替代来源，再生医学疗法可能会在临床上找到针对性的应用。尽管这些细胞已被证明表达特定的间充质标记物，并能在特定的培养条件下分化为间充质谱系，但在相同的标准化培养环境下比较研究中，确定这些细胞的产量和分化潜力仍然至关重要。此外，有机会利用多器官供体的骨髓 (BM) 中的 MSC 进行细胞储存也具有重要意义。为了评估替代 MSC 来源的相对潜力，我们使用标准化的分离和培养方案，分析和比较了来源于尸体脂肪和 BM 组织以及产后附属物（胎盘和脐带）的人 MSC 的产量和分化潜力。与其他组织来源相比，BM 中含有大量单核细胞 (MNCs)。尽管如此，这些细胞需要更高的细胞接种密度才能成功分离 MSC。MNC 群体高度异质，表达不同的 MSC 标记物，并且个体间存在很大差异。通过组织培养塑料黏附进行 MSC 选择后，细胞表现出相似的增殖能力，具有不同的集落形态，并对一组典型的 MSC 标记物呈阳性。体外分化试验表明，脂肪组织和 BM MSC 的成骨分化能力更高，而 BM MSC 的软骨分化能力更高。
