Zeng Ya-Wen, Du Juan, Yang Shu-Ming, Pu Xiao-Ying, Wang Yu-Chen, Yang Tao, Sun Zheng-Hai, Xin Pei-Yao
Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences/Agricultural Biotechnology Key Laboratory of Yunnan Province, Kunming 650205, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2010 Dec;30(12):3388-94.
The zonal characteristics and cultivated types difference of functional components in brown rice of 907 accessions for primary core collection for rice landrace from 16 prefectures of five rice regions in Yunnan Province were determined by ultraviolet spectrophotometry of DU640 type from BECKMAN. The analytical results showed that the contents mg x (100 g)(-1) of total flavone in brown rice is 306.98 +/- 192.75, the content of upland (341.74 +/- 185.11) is the most significantly higher than that of lowland (290.41 +/- 193.72), that of the glutinous (315.54 +/- 197.64) is significantly higher than non-glutinous (171.68 +/- 11.76), that of early-mid (318.25 +/- 197.93) is the most significantly higher than late (282.12 +/- 178.11), that of red rice (379.22 +/- 197.70) and purple rice (365.61 +/- 195.44) are the most significantly higher than white rice (216.96 +/- 142.11), and that of nuda (332.68 +/- 196.22) is significantly higher than non-nuda (300. 48 +/- 191.14). Among five rice regions, in turn III (327.13) > LL (324.23) > IV (273.11) > V (270.16) > I (258.26), namely it is the most significantly highest (p < 0.01) for contents of total flavone from South Yunnan (II, III) than that of middle Yunnan; It is the most significantly highest from Simao prefecture than that of 8 prefectures, but it is the most significantly low from Baoshan prefecture than that of 7 prefectures. The contents mg x (100 g)(-1) of g-amino butyric acid (GABA) in brown rice is 7.43 +/- 2.53, the content of lowland (7.59 +/- 2. 56) is the most significantly higher than that of upland (7.09 +/- 2.45), that of non-glutinous (8.55 +/- 2.88) is the most significantly higher than glutinous (7.10 +/- 2.32), that of late (7.88 +/- 2.64) is the most significantly higher than early-mid (7.23 +/- 2.45), that of white rice (8.38 +/- 2.66) is the most significantly higher than red rice (6.63 +/- 2.14) and purple rice (7.34 +/- 2.18); Among five rice regions in turn II (7.69) > I (7.40) > IV (7.39) > III (7.33) > V (6.64), viz. the content of GABA from South Yunnan single/double cropping rice region is clearly higher than that of Northwest Yunnan cold highland japonica rice region; It is significant difference of GABA content in brown rice for that Simao prefecture South Yunnan and Yuxi as well as Baoshan prefecture, at least than that of 5 prefectures. The contents (%) of resistant starch in brown rice is 0.75 +/- 0.29, the contents of indica (0.78 +/- 0.35) is significantly higher than japonica (0.74 +/- 0.24), that of glutinous (0.78 +/- 0.31) is the most significantly higher than non-glutinous (0. 67 +/- 0.22), that of late (0.77 +/- 0. 35) is the most significantly higher than early-mid (0.75 +/- 0.26), that of red rice (0.81 +/- 0.30) and purple rice (0.70 +/- 0.30) is the most significantly higher than white rice (0.69 +/- 0.27); Among five rice regions in turn I (0.83) > II (0.79) > III (0.76) > V (0. 55) > IV (0.50), namely it is the highest (p < 0.01) for contents of resistant starch from the middle and South Yunnan province (I, II, III) than that of northern rice regions (V, IV), and the lowest for contents of resistant starch of Lijiang prefecture of northwestern and Zaotong of northeastern in this province compared to that of 13 prefectures except Diqing prefectures. These results not only reveal the most significant difference between lowland versus upland, glutinous versus nonglutinous, early/mid rice and red/purple versus white rice based on the contents of total flavone and resistant starch as well as g-amino butyric acid (GABA) in brown rice (p < 0.01), and no significant difference between indica and japonica, awn and no-awn, and common rice and aromatic/soft rice, but also elucidate obvious zonal characteristics of three functional components in brown rice of Yunnan rice. The above results provided theory bases for the genetic breeding and production of functional rice and solve the problems of chronic for human being.
采用BECKMAN公司DU640型紫外可见分光光度计,对云南省5个稻作区16个州(市)的907份稻种资源初级核心种质糙米中功能成分的地带性特征及栽培类型差异进行了测定。分析结果表明,糙米中总黄酮含量为306.98±192.75mg/100g,陆稻(341.74±185.11)显著高于水稻(290.41±193.72),糯稻(315.54±197.64)显著高于非糯稻(171.68±11.76),中早熟品种(318.25±197.93)显著高于晚熟品种(282.12±178.11),红米(379.22±197.70)和紫米(365.61±195.44)显著高于白米(216.9~6±142.11),裸粒稻(332.68±196.22)显著高于非裸粒稻(300.48±191.14)。在5个稻作区中,依次为Ⅲ区(327.13)>Ⅱ区(324.23)>Ⅳ区(273.11)>Ⅴ区(270.16)>Ⅰ区(258.26),即滇南(Ⅱ、Ⅲ)糙米总黄酮含量显著高于滇中;思茅地区显著高于8个州(市),而保山地区显著低于7个州(市)。糙米中γ-氨基丁酸(GABA)含量为7.43±2.53mg/100g,水稻(7.59±~6±2.56)显著高于陆稻(7.09±2.45),非糯稻(8.55±2.88)显著高于糯稻(7.10±2.32),晚熟品种(7.88±2.64)显著高于中早熟品种(7.23±2.45),白米(8.38±2.66)显著高于红米(6.63±2.14)和紫米(7.34±2.18);在5个稻作区中,依次为Ⅱ区(7.69)>Ⅰ区(7.40)>Ⅳ区(7.39)>Ⅲ区(7.33)>Ⅴ区(6.64),即滇南单双季稻区GABA含量明显高于滇西北高寒粳稻区;滇南思茅地区及玉溪、保山地区糙米GABA含量与至少5个州(市)差异显著。糙米中抗性淀粉含量为0.75±0.29%,籼稻(0.78±0.35)显著高于粳稻(0.74±0.24),糯稻(0.78±0.31)显著高于非糯稻(0.67±0.22),晚熟品种(0.77±0.35)显著高于中早熟品种(0.75±0.26),红米(0.81±0.30)和紫米(0.70±0.30)显著高于白米(0.69±0.27);在5个稻作区中,依次为Ⅰ区(0.83)>Ⅱ区(0.79)>Ⅲ区(0.76)>Ⅴ区(0.55)>Ⅳ区(0.50),即滇中及滇南(Ⅰ、Ⅱ、Ⅲ)糙米抗性淀粉含量显著高于滇北稻区(Ⅴ、Ⅳ),滇西北丽江地区及滇东北昭通地区糙米抗性淀粉含量在省内除迪庆州外13个州(市)中最低。这些结果不仅揭示了糙米中总黄酮、抗性淀粉及γ-氨基丁酸(GABA)含量在水稻与陆稻、糯稻与非糯稻、中早熟与晚熟、红米/紫米与白米之间差异极显著(p<0.01),而籼粳、有无芒、普通与香软米之间差异不显著,而且阐明了云南稻糙米中3种功能成分具有明显的地带性特征。上述结果为功能稻的遗传育种和生产提供了理论依据,对解决人类健康问题具有重要意义。