Yang Shu-Min, Jiang Ze-Hui, Ren Hai-Qing, Fei Ben-Hua, Liu Xing-E
Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2010 Dec;30(12):3399-404.
The lignin distribution in different anatomical regions of developing moso bamboo Phyllostachys pubescens f. lutea Wen was investigated by means of optical microscope and visible-light microspectrophotometry coupled with the Wiesner and Maule reaction. The lignin is widely distributed in different tissues, in which there is a difference in lignin content between different ages, tissues and anatomical regions. Guaiacyl lignin and Syringyl lignin unit can be found in cell wall of fibre, parenchyma and vessel. Lignin content of secondary wall, cell corner and compound middle lamellae of fibre increased gradually within 12 months, then almost remained in the same level or decreased slowly in the culms with an age from 12 to 78 months. The lignification rate in secondary wall, cell corner and compound middle lamellae of fibre changed with age until they reached a relative steady value. There is no regular variation in lignin content with different radial location of culms and different location in fibre strands within one age. The secondary wall, compound middle lamellae of parenchyma and vessel secondary wall lignified rapidly within 12 months, and then changed slowly.
采用光学显微镜、可见光显微分光光度法结合Wiesner反应和Maule反应，对发育中的毛竹Phyllostachys pubescens f. lutea Wen不同解剖区域的木质素分布进行了研究。木质素广泛分布于不同组织中，不同年龄、组织和解剖区域的木质素含量存在差异。在纤维、薄壁组织和导管的细胞壁中可发现愈创木基木质素和紫丁香基木质素单元。在12个月内，纤维次生壁、细胞角隅和复合中层的木质素含量逐渐增加，随后在12至78个月龄的竹秆中几乎保持在同一水平或缓慢下降。纤维次生壁、细胞角隅和复合中层的木质化速率随年龄变化，直至达到相对稳定值。在同一龄期内，竹秆不同径向位置和纤维束内不同位置的木质素含量没有规律变化。薄壁组织的次生壁、复合中层以及导管次生壁在12个月内迅速木质化，然后变化缓慢。
