原位杂交检测同源盒基因在口腔鳞状细胞癌中呈现出不同的表达模式。
In situ hybridization detection of homeobox genes reveals distinct expression patterns in oral squamous cell carcinomas.
机构信息
Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
出版信息
Histopathology. 2011 Jan;58(2):225-33. doi: 10.1111/j.1365-2559.2011.03751.x.
AIMS
To analyse the expression of three homeobox genes (HOXA7, PITX1 and PRRX1) in oral squamous cell carcinomas (OSCC) and the relationship of such expression to certain distinct histopathological features of OSCC and in comparison to adjacent non-neoplastic epithelium (NT).
METHODS AND RESULTS
Digoxigenin-labelled riboprobes that are specific for each homeobox gene were generated and in situ hybridization was carried out on frozen sections. In NT samples, HOXA7 and PITX1 transcripts were found more frequently in all epithelial layers, while PRRX1 was expressed in the basal layer. With OSCC samples, expression of the three genes was associated with all histological features. However, the HOXA7 and PITX1 signals were more intense in sheets and nests and PRRX1 in small nests and isolated cells.
CONCLUSION
HOXA7, PIXT1 and PRRX1 homeobox genes have different patterns of expression in OSCC depending on its histological features.
目的
分析三个同源盒基因（HOXA7、PITX1 和 PRRX1）在口腔鳞状细胞癌（OSCC）中的表达情况，并探讨其表达与 OSCC 某些特定组织病理学特征的关系，以及与相邻非肿瘤上皮（NT）的关系。
方法和结果
针对每个同源盒基因生成了 DIG 标记的 RNA 探针，并对冰冻切片进行了原位杂交。在 NT 样本中，HOXA7 和 PITX1 转录本更频繁地出现在所有上皮层中，而 PRRX1 则在上皮层表达。在 OSCC 样本中，这三个基因的表达与所有组织学特征有关。然而，HOXA7 和 PITX1 的信号在片状和巢状结构中更为强烈，而 PRRX1 则在小巢状结构和孤立细胞中表达。
结论
HOXA7、PITX1 和 PRRX1 同源盒基因在 OSCC 中根据其组织病理学特征呈现不同的表达模式。
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