Department of Surgical Sciences, Oral and Maxillo-facial Surgery, Medical Faculty Uppsala, SE-751 85 Uppsala, Sweden.
Anticancer Res. 2011 Oct;31(10):3345-51.
Previously we used microarray genomic hybridization technology to explore genome-wide profiles of chromosomal aberrations in samples of oral squamous cell carcinomas (OSCCs) and paired normal controls. Based on these findings, 9 genes related to apoptosis, cell cycle regulation and intermediate filament proteins were selected and their differential expression status was examined by real-time quantitative RT-PCR in 26 samples of Sudanese OSCCs and their matched normal controls. The findings were correlated with the habit of toombak use. The mRNA levels of Bcl2, keratin 1, keratin 13 and p53 were significantly lower and the level of survivin was significantly higher in the OSCC samples of the toombak users compared to their paired control samples. A significant down-regulation in keratin 1 and keratin 13 expression levels was found in the OSCC samples of the nontoombak users compared to their normal control samples. The differential expression of genes related to apoptosis, cell cycle regulation and types I and II keratin could be useful diagnostic markers and provide valuable information for the understanding of oral malignancy in relation to toombak use.
先前，我们运用微阵列基因组杂交技术，探索了口腔鳞状细胞癌（OSCC）样本及其配对正常对照中染色体畸变的全基因组图谱。基于这些发现，选择了 9 个与凋亡、细胞周期调控和中间丝蛋白相关的基因，并通过实时定量 RT-PCR 检测了苏丹口腔鳞状细胞癌 26 例及其配对正常对照中这些基因的差异表达情况。研究结果与使用土库曼的习惯相关联。与配对的正常对照组相比，使用土库曼的口腔鳞状细胞癌患者样本中 Bcl2、角蛋白 1、角蛋白 13 和 p53 的 mRNA 水平显著降低，而 survivin 水平显著升高。与配对的正常对照组相比，非土库曼使用者的口腔鳞状细胞癌患者样本中角蛋白 1 和角蛋白 13 的表达水平显著下调。与凋亡、细胞周期调控以及 I 型和 II 型角蛋白相关的基因的差异表达可能是有用的诊断标志物，并为了解与土库曼使用相关的口腔恶性肿瘤提供了有价值的信息。
