滋养层栓子向母体肺的迁移：母体血液中无细胞DNA的一个来源？
Deportation of trophoblastic emboli to maternal lung: A source of cell-free DNA in maternal blood?
作者信息
Benirschke Kurt, Willes Lawrence
机构信息
Professor of Pathology and Reproductive Medicine; Resident in Pathology; UCSD; Department of Pathology; University of California Medical Center; San Diego, CA USA.
出版信息
Chimerism. 2010 Jul-Sep;1(1):15-8. doi: 10.4161/chim.1.1.12140.
During human pregnancy there is a continuous transport of numerous syncytiotrophoblastic cells from the intervillous space of the placenta into the maternal lung. There these cells undergo apoptosis and the fetal nuclear DNA is liberated within the pulmonary capillaries to become cffDNA in the maternal serum. We have examined the sections of lungs of 11 pregnant women (from 8 weeks to term gestation) who had come to the Medical Examiner's Offices after their traumatic demise. We then identified the deported, embolized trophoblastic cells in pulmonary capillaries and attempted to show them to contain hCG immunohistochemically but were unable to do this. We also determined their apoptotic profiles by TUNEL reaction.
在人类妊娠期间，大量合体滋养层细胞持续从胎盘绒毛间隙转运至母体肺部。在肺部，这些细胞发生凋亡，胎儿核DNA在肺毛细血管内释放，成为母体血清中的游离胎儿DNA（cffDNA）。我们检查了11名孕妇（妊娠8周直至足月）在遭遇创伤性死亡后送至法医办公室的肺部切片。然后我们在肺毛细血管中识别出被驱逐、栓塞的滋养层细胞，并试图通过免疫组织化学方法显示它们含有hCG，但未能成功。我们还通过TUNEL反应确定了它们的凋亡情况。
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