用于检测胃活检标本中幽门螺杆菌的巢式聚合酶链反应

Nested polymerase chain reaction for detection of Helicobacter pylori in gastric biopsy specimens.

作者信息

Ottiwet Orawan, Chomvarin Chariya, Chaicumpar Kunyaluk, Namwat Wises, Mairiang Pisaln

机构信息

Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.

出版信息

Southeast Asian J Trop Med Public Health. 2010 Nov;41(6):1423-31.

PMID:21329319

Abstract

Sensitivity and specificity are important for tests used to defect Helicobacter pylori infection from gastric biopsy specimens. Molecular methods, such as PCR and nested PCR, are sensitive methods for H. pylori detection. The objective of this study was to evaluate the performance of PCR and nested PCR compared to culture, the rapid urease test (RUT) and histology for the diagnosis of H. pylori in 130 gastric biopsy specimens from symptomatic dyspeptic patients. Sensitivity and specificity with PCR were 91 and 100% and with nested PCR were 95 and 97%, respectively. H. pylori was detected by PCR and nested PCR at levels as low as 125 fg (70 cells) and 25 fg (14 cells), respectively. These results suggest nested PCR is a highly sensitive direct method to detect H. pylori infection from biopsy specimens.

摘要

敏感性和特异性对于用于从胃活检标本中检测幽门螺杆菌感染的检测方法很重要。分子方法，如聚合酶链反应（PCR）和巢式PCR，是检测幽门螺杆菌的敏感方法。本研究的目的是评估PCR和巢式PCR与培养、快速尿素酶试验（RUT）和组织学方法相比，在诊断130例有症状消化不良患者的胃活检标本中幽门螺杆菌的性能。PCR的敏感性和特异性分别为91%和100%，巢式PCR的敏感性和特异性分别为95%和97%。通过PCR和巢式PCR分别在低至125fg（70个细胞）和25fg（14个细胞）的水平检测到幽门螺杆菌。这些结果表明巢式PCR是一种从活检标本中检测幽门螺杆菌感染的高度敏感的直接方法。