De Lello E, Boulard C
Institute of Biosciences, UNESP, Campus de Botucatu, Brazil.
Med Vet Entomol. 1990 Jul;4(3):303-9. doi: 10.1111/j.1365-2915.1990.tb00444.x.
The three larval stages of Dermatobia hominis (Linnaeus) have been evaluated for their immunogenicity by ELISA and immunodiffusion (ID) using sera from experimentally infested rabbits. During a primary infestation, first instar D. hominis were found to cause most reaction and allowed the earliest diagnosis by ELISA. An inhibition of the antibody response against second and third instars was observed. The inhibition disappeared after departure of the larvae from the host. In experimentally immunized hosts the antibody response, following challenge, was highest against second and third instar antigens. Antibody remained elevated during the infestation but fell immediately after the larvae had left the host.
利用实验感染兔子的血清，通过酶联免疫吸附测定（ELISA）和免疫扩散（ID）对人皮蝇（Linnaeus）的三个幼虫阶段的免疫原性进行了评估。在初次感染期间，发现第一龄期人皮蝇引起的反应最大，并且通过ELISA能够最早诊断出来。观察到针对第二和第三龄期幼虫的抗体反应受到抑制。幼虫离开宿主后，这种抑制作用消失。在实验免疫的宿主中，攻击后针对第二和第三龄期幼虫抗原的抗体反应最高。在感染期间抗体水平持续升高，但幼虫离开宿主后立即下降。
