Oliveira-Sequeira T C, Sequeira J L, Schmitt F L, De Lello E
Departamento de Parasitologia, Universidade Estadual Paulista, Brazil.
Med Vet Entomol. 1996 Oct;10(4):323-30. doi: 10.1111/j.1365-2915.1996.tb00752.x.
Six cattle that had earlier exposure to Dermatobia hominis were infested experimentally with first-instar larvae of the parasite. Skin biopsies taken at intervals were studied in wax and in plastic sections. The avidin-biotin-peroxidase method was used to detect the presence and localization of host immunoglobulins (Igs) G and M and antigens of first and second instar larvae of Dermatobia hominis. The larvae penetrated actively through the skin and migrated towards the subcutaneous tissues. The great numbers of eosinophils suggest that they are the most important cell in mediating damage to D. hominis larvae. The immunoglobulins bound only to dead or moulting larvae in which access to binding sites may have been altered. This could represent a morphological manifestation of a mechanism that protects larvae from the host immune response. Large amounts of soluble antigens detected along the fistulous tract may be important in the maintenance of this tract by distributing the normal cicatrization process.
六头先前接触过人皮蝇的牛被实验性地感染了该寄生虫的一龄幼虫。定期采集皮肤活检组织，制成石蜡切片和塑料切片进行研究。采用抗生物素蛋白-生物素-过氧化物酶法检测宿主免疫球蛋白（Ig）G和M以及人皮蝇一龄和二龄幼虫抗原的存在和定位。幼虫主动穿透皮肤并向皮下组织迁移。大量嗜酸性粒细胞表明它们是介导对人皮蝇幼虫损伤的最重要细胞。免疫球蛋白仅与死亡或正在蜕皮的幼虫结合，其结合位点可能已发生改变。这可能代表了一种保护幼虫免受宿主免疫反应的机制的形态学表现。沿着瘘管检测到的大量可溶性抗原可能通过分散正常的瘢痕形成过程在维持该瘘管方面起重要作用。
