Bacterial Diseases Programme, Medical Research Council Laboratories, PO Box 273, Banjul, The Gambia.
Int J Infect Dis. 2011 Apr;15(4):e282-8. doi: 10.1016/j.ijid.2010.12.011. Epub 2011 Feb 16.
To understand the pattern of immune responses to pneumococcal proteins during invasive disease as a guide to their development as vaccine candidates.
The antibody concentration and avidity, as well as frequency of interferon-gamma (IFN-γ)-, interleukin-10 (IL-10)-, and tumor necrosis factor-alpha (TNF-α)-containing CD4+ T-lymphocytes in response to pneumolysin, pneumococcal surface protein A (PspA), and choline-binding protein A (CbpA), during and after invasive pneumococcal disease (IPD) in 20 children were compared to those of 20 healthy matched controls.
During the acute phase of IPD, the concentrations of antibodies against these three pneumococcal proteins were lower, whereas the frequencies of IL-10- and TNF-α-producing CD4+ T-cells were higher, compared to values obtained during convalescence and in healthy controls (p < 0.01). In addition, the concentrations of antibodies against the capsular polysaccharides for the serotypes isolated from these patients, were all below the detection level of the assay during both the acute and convalescent phases of IPD.
These data indicate that the recognition of these antigens by the immune system occurs in variable proportions according to the stage of infection, implying the important role of these in the pathogenesis of IPD, and support their usefulness in vaccine development.
了解侵袭性疾病期间对肺炎球菌蛋白的免疫反应模式，以此作为将其开发为疫苗候选物的指导。
比较 20 例侵袭性肺炎球菌病（IPD）患儿和 20 例健康匹配对照者在 IPD 期间和之后对肺炎球菌溶血素、肺炎球菌表面蛋白 A（PspA）和胆碱结合蛋白 A（CbpA）产生抗体的浓度和亲和力以及含干扰素-γ（IFN-γ）、白细胞介素-10（IL-10）和肿瘤坏死因子-α（TNF-α）的 CD4+T 淋巴细胞的频率。
与恢复期和健康对照组相比，在 IPD 的急性阶段，针对这三种肺炎球菌蛋白的抗体浓度较低，而产生 IL-10 和 TNF-α的 CD4+T 细胞的频率较高（p<0.01）。此外，在 IPD 的急性和恢复期，患者分离的血清型荚膜多糖的抗体浓度均低于测定的检测下限。
这些数据表明，免疫系统对这些抗原的识别根据感染阶段以不同的比例发生，这意味着它们在 IPD 的发病机制中具有重要作用，并支持它们在疫苗开发中的有用性。
