Scully F E, Mazina K E, Ringhand H P, Chess E K, Campbell J A, Johnson J D
Department of Chemical Sciences, Old Dominion University, Norfolk, Virginia 23529-0126.
Chem Res Toxicol. 1990 Jul-Aug;3(4):301-6. doi: 10.1021/tx00016a005.
Amino acids were identified and quantified in the stomach fluid recovered from animals fasted 8, 24, and 48 h. After in vitro chlorination of stomach fluid, three chloramino acids, N-chloroglycine, either N-chloroleucine or N-chloroisoleucine, and N-chlorophenylalanine, were identified by GC/MS analysis of dansylated methyl ester derivatives. Formation of N-chlorovaline and N-chloroserine was suggested by their HPLC retention times, but their identification could not be confirmed by GC/MS data.
对禁食8小时、24小时和48小时的动物所回收的胃液中的氨基酸进行了鉴定和定量分析。胃液经体外氯化处理后，通过对丹磺酰化甲酯衍生物的气相色谱/质谱分析，鉴定出三种氯代氨基酸，即N-氯代甘氨酸、N-氯代亮氨酸或N-氯代异亮氨酸，以及N-氯代苯丙氨酸。根据高效液相色谱保留时间推测出了N-氯代缬氨酸和N-氯代丝氨酸的形成，但气相色谱/质谱数据无法证实它们的存在。
