Abate C, Curran T
Department of Molecular Oncology & Virology, Roche Institute of Molecular Biology, Nutley, NJ 07110.
Semin Cancer Biol. 1990 Feb;1(1):19-26.
The nuclear proto-oncogenes, c-fos and c-jun, are induced in response to a diverse array of extracellular stimuli. Their protein products, Fos and Jun, form a heterodimeric complex that interacts with the DNA regulatory element known as the AP-1 binding site. Protein dimerization occurs via a parallel interaction of leucine zipper domains and is required for DNA binding. In addition to the leucine zipper, DNA binding requires two clusters of basic amino acids adjacent to the leucine zipper domains of both Fos and Jun. The leucine zipper and DNA-binding regions are highly conserved among the c-fos and c-jun families of related inducible genes. Thus, multiple protein complexes can be formed that may interact with AP-1 binding sites in numerous genes to affect gene expression in response to environmental signals.
核原癌基因c-fos和c-jun可因多种细胞外刺激而被诱导。它们的蛋白质产物Fos和Jun形成一种异二聚体复合物，该复合物与被称为AP-1结合位点的DNA调控元件相互作用。蛋白质二聚化通过亮氨酸拉链结构域的平行相互作用发生，并且是DNA结合所必需的。除亮氨酸拉链外，DNA结合还需要Fos和Jun的亮氨酸拉链结构域附近的两簇碱性氨基酸。亮氨酸拉链和DNA结合区域在相关诱导基因的c-fos和c-jun家族中高度保守。因此，可以形成多种蛋白质复合物，这些复合物可能与众多基因中的AP-1结合位点相互作用，以响应环境信号影响基因表达。
