Philips Research Laboratories, 101 Cambridge Science Park, Milton Road, Cambridge CB4 0FY, UK.
Lab Chip. 2011 Apr 7;11(7):1249-55. doi: 10.1039/c0lc00587h. Epub 2011 Feb 17.
Counting the different subpopulations of cells in a fingerprick of human blood is important for a number of clinical point-of-care (PoC) applications. It is a challenge to demonstrate the integration of sample preparation and detection techniques in a single platform. In this paper we demonstrate a generic microfluidic platform that combines sample processing and characterisation and enumeration in a single, integrated system. Results of microfluidic 3-part differential leukocyte (granulocyte, lymphocyte and monocyte) counts, together with erythrocyte and thrombocyte (platelet) counts, in human blood are shown and corroborated with results from hospital clinical laboratory analysis.
在人体血液的一次指尖采血样中计算不同的细胞亚群对于许多临床即时检测（PoC）应用非常重要。在单个平台上展示样品制备和检测技术的集成是一项挑战。在本文中，我们展示了一种通用的微流控平台，该平台将样品处理和特征化以及在单个集成系统中的计数相结合。展示了微流控三分群白细胞（粒细胞、淋巴细胞和单核细胞）计数的结果，以及人血中的红细胞和血小板（血小板）计数，并与医院临床实验室分析的结果相吻合。
