Department of Pharmaceutics, Virginia Commonwealth University Medical Center, PO Box 980533, Richmond, VA 23298-0533, USA.
Anal Bioanal Chem. 2011 Apr;400(1):237-44. doi: 10.1007/s00216-011-4740-x. Epub 2011 Feb 18.
A in-line desorption device was developed, which allows for direct analysis of dried blood spots eliminating the need for punching disks from the filter paper cards. Using this device, we have validated a method to quantify biomarkers related to maple syrup urine disease (MSUD), a metabolism disorder that often requires a second-tier test for confirmation. Direct analysis of newborn screening cards is conducted in-line with a high-resolution chromatographic separation with mass spectrometry using electrospray ionization and multiple-reaction monitoring. Quantification of leucine and isoleucine using an isotopically labeled internal standard encompasses a range suitable for MSUD assessment. Precision and accuracy of the technique was acceptable with relative standard deviations within 10% at three fortified concentrations and an unfortified level. A post-column infusion test shows minimum matrix suppression was observed using this direct sampling technique.
我们开发了一种在线解吸装置，它允许直接分析干血斑，从而无需从滤纸卡上打孔。使用该装置，我们已经验证了一种定量分析与枫糖尿症（MSUD）相关生物标志物的方法，MSUD 是一种代谢紊乱疾病，通常需要进行二级测试来确认。直接分析新生儿筛查卡，与使用电喷雾电离和多反应监测的高分辨率色谱分离进行在线分析。使用同位素标记的内标定量分析亮氨酸和异亮氨酸，涵盖了适合 MSUD 评估的范围。该技术的精密度和准确度可以接受，在三个加标浓度和未加标水平下，相对标准偏差在 10%以内。柱后注入测试表明，使用这种直接采样技术观察到最小的基质抑制。
