Shandong University, Jinan, People's Republic of China.
Neurol Sci. 2011 Aug;32(4):589-94. doi: 10.1007/s10072-011-0486-7. Epub 2011 Feb 18.
Current theory or hypothesis relevant to migraine indicates that trigeminovascular system plays a pivotal role in the pathophysiology of migraine. Particularly, release of neuropeptide and induction of c-fos like immunoreactivity (c-fos LI) within trigeminal nucleus caudalis neurons are regarded as activation markers of trigeminovascular system. In the present study, we set up a rat model for migraine triggered by nitroglycerin (NTG) and coagulated the middle meningeal artery by heating. Using this model, we determined the plasma calcitonin gene-related peptide (CGRP) level as well as the expression of c-fos in trigeminal nucleus caudalis of rats. We found that NTG led to markedly increase in plasma CGRP level and c-fos expression in trigeminal nucleus caudalis compared with the isotonic saline-treated group (P < 0.05). More importantly, heat coagulation of middle meningeal artery could decrease plasma CGRP level and c-fos expression in trigeminal nucleus caudalis (P < 0.05). Heat coagulation of middle meningeal artery may ameliorate sufferings of rat induced by NTG and play an important role in restraining the release of CGRP as well as the activation of neurons in trigeminal nucleus caudalis in rats following NTG infusion.
当前与偏头痛相关的理论或假说表明，三叉血管系统在偏头痛的病理生理学中起着关键作用。特别是，三叉神经脊核内神经肽的释放和 c-fos 样免疫反应性（c-fos LI）的诱导被认为是三叉血管系统激活的标志。在本研究中，我们建立了一个由硝酸甘油（NTG）触发的偏头痛大鼠模型，并通过加热凝固脑膜中动脉。使用该模型，我们测定了大鼠血浆降钙素基因相关肽（CGRP）水平以及三叉神经脊核内 c-fos 的表达。结果发现，与生理盐水处理组相比，NTG 导致大鼠血浆 CGRP 水平和三叉神经脊核内 c-fos 表达显著增加（P<0.05）。更重要的是，脑膜中动脉加热凝固可降低三叉神经脊核内的血浆 CGRP 水平和 c-fos 表达（P<0.05）。脑膜中动脉加热凝固可减轻 NTG 诱导的大鼠疼痛，并在 NTG 输注后抑制大鼠三叉神经脊核内 CGRP 的释放和神经元的激活方面发挥重要作用。
