School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
J Med Food. 2011 Mar;14(3):247-56. doi: 10.1089/jmf.2009.1389.
Culinary herbs and spices have been widely used for their hypoglycemic, lipid-lowering, and anti-inflammatory activities. This study examined the physiologic activity of hydrophilic components using extracts of turmeric or laurel leaf powder. Aqueous extracts of turmeric and laurel showed potent inhibitory activity against fructose-mediated glycation with antioxidant ability against low-density lipoprotein (LDL) oxidation and radical scavenging activity. The turmeric and laurel extracts had potent cholesteryl ester transfer protein (CETP) inhibitory ability (up to 23% and 40% inhibition, respectively) at a final concentration of 10 μg/mL. The turmeric and laurel extracts inhibited the cellular uptake of oxidized LDL into macrophages, which is the initial step in atherogenesis. For in vivo testing, zebrafish consumed a high cholesterol diet (HCD) (final concentration, 4% [wt/wt]) with or without turmeric or laurel powder (final concentration, 10% [wt/wt]). The turmeric and laurel groups had a 14% and 12% decrease, respectively, in the weight and height ratios compared to the HCD group. The plasma total cholesterol level was significantly lower in the turmeric and laurel groups (48% and 28% less, respectively, than in the HCD group). Plasma triglycerides were more markedly reduced in the turmeric and laurel groups than in the HCD group (68% and 56% less, respectively, than the HCD group). In conclusion, the hydrophilic extracts of turmeric and laurel potently suppressed the incidence of atherosclerosis via a strong antioxidant potential, prevention of apolipoprotein A-I glycation and LDL phagocytosis, and inhibition of CETP. Consumption of turmeric and laurel extracts exhibited hypolipidemic and antioxidant activities in a hypercholesterolemic zebrafish model.
食用香草和香料因其具有降血糖、降血脂和抗炎活性而被广泛应用。本研究使用姜黄或月桂叶粉提取物来检测亲水性成分的生理活性。姜黄和月桂的水提取物对果糖介导的糖化具有很强的抑制活性，具有对抗低密度脂蛋白 (LDL) 氧化的抗氧化能力和自由基清除活性。姜黄和月桂提取物在 10μg/mL 的终浓度下对胆固醇酯转移蛋白 (CETP) 具有很强的抑制能力（分别高达 23%和 40%的抑制作用）。姜黄和月桂提取物抑制氧化 LDL 被巨噬细胞摄取，这是动脉粥样硬化形成的初始步骤。在体内试验中，斑马鱼食用高胆固醇饮食（HCD）（终浓度为 4%[wt/wt]），并添加或不添加姜黄或月桂粉（终浓度为 10%[wt/wt]）。与 HCD 组相比，姜黄组和月桂组的体重与身高比分别降低了 14%和 12%。姜黄组和月桂组的血浆总胆固醇水平显著降低（分别比 HCD 组低 48%和 28%）。姜黄组和月桂组的血浆甘油三酯水平比 HCD 组明显降低（分别比 HCD 组低 68%和 56%）。综上所述，姜黄和月桂的亲水性提取物通过强大的抗氧化潜力、抑制载脂蛋白 A-I 糖化和 LDL 吞噬以及抑制 CETP，有力地抑制了动脉粥样硬化的发生。在高胆固醇血症斑马鱼模型中，姜黄和月桂提取物具有降血脂和抗氧化活性。
