In vitro Brasil Ltda. Mogi Mirim SP, 13800-970 Brazil.
Theriogenology. 2011 Jun;75(9):1640-6. doi: 10.1016/j.theriogenology.2010.12.026. Epub 2011 Feb 18.
The objective was to clarify in vitro production of bovine embryos in Brazil. Data from 656 ovum pick-up/in vitro production (OPU/IVP) procedures, performed on 317 Nelore (Bos indicus) donors, without hormone stimulation or control of ovarian follicular waves, were analysed. Donors were subjected to OPU from one to nine times (no specific schedule), with < 15 d between consecutive procedures. There were 20,848 oocytes, of which 15,747 (75.53%) were considered viable, 5,446 embryos were obtained, 5,398 embryos were immediately transferred, resulting in 1,974 pregnancies (36.57%) at Day 30 and 1,788 (33.12%) pregnancies at Day 60. The average number of total and viable oocytes produced per OPU session was (mean ± SEM) 30.84 ± 0.88 and 23.35 ± 0.7 (average of 8.1 ± 0.3 embryos and 3.0 ± 0.1 pregnancies per OPU-IVP procedure). Since oocyte production varied widely among donor, they were designated as very high, high, intermediate, and low, with 58.94 ± 2.04, 32.61 ± 0.50, 22.13 ± 0.50, and 10.26 ± 0.57 oocytes, respectively, produced by 78, 80, 79, and 80 donors. The number of viable oocytes recovered ranged from 0 to 128; since donors with numerous viable oocytes produced many viable embryos and pregnancies, oocyte production was useful for donor selection. However, there was no significant effect of the number of OPU sessions per donor on mean numbers of oocytes produced. In conclusion, we confirmed field reports of high oocyte production by some Nelore donors and demonstrated individual variation in oocyte yield, which was associated with embryo production and pregnancy rates.
本研究旨在阐明巴西牛胚胎的体外生产情况。对 317 头尼洛拉（Bos indicus）供体进行了 656 次卵母细胞抽吸/体外生产（OPU/IVP）操作的数据进行了分析，这些供体未接受激素刺激或卵巢卵泡波的控制，也没有特定的时间安排，连续操作之间的间隔时间<15d。共获得 20848 个卵母细胞，其中 15747 个（75.53%）被认为具有活力，获得 5446 个胚胎，其中 5398 个胚胎立即进行了移植，第 30 天获得 1974 例（36.57%）妊娠，第 60 天获得 1788 例（33.12%）妊娠。每次 OPU 获得的总卵母细胞和有活力的卵母细胞数量分别为（平均值±SEM）30.84±0.88 和 23.35±0.7（平均每个 OPU-IVP 操作产生 8.1±0.3 个胚胎和 3.0±0.1 个妊娠）。由于供体间卵母细胞产量差异很大，将其分为极高、高、中、低四个等级，分别有 58.94±2.04、32.61±0.50、22.13±0.50 和 10.26±0.57 个卵母细胞，其中 78、80、79 和 80 个供体分别属于这四个等级。可回收的有活力卵母细胞数量范围为 0 至 128；由于具有大量有活力卵母细胞的供体产生了许多有活力的胚胎和妊娠，因此卵母细胞产量可用于供体选择。然而，每个供体的 OPU 操作次数对卵母细胞产量平均值没有显著影响。总之，本研究证实了一些尼洛拉供体具有高产卵母细胞的田间报告，并证明了卵母细胞产量存在个体差异，这与胚胎产量和妊娠率有关。
