Kulpiisova Altyn, Yessengaliyev Kairly, Kassimova Gulsara, Kozhakhmetova Ainat, Kadraliyeva Bakytkanym, Rustem Abeldinov, Temirzhanova Alma, Burambayeva Nadezhda, Chylbak-Ool Salbak, Pakhomova Elena, Abekeshev Nurzhan, Baikadamova Gulnara, Kemeshev Zhomart, Tegza Alexandra, Issimov Arman, White Peter
Department of Veterinary Medicine, A. Baitursynov Kostanay Regional University, Kostanay 110000, Kazakhstan.
Department of Veterinary Medicine, Zhangir Khan West Kazakhstan Agrarian Technical University, Oral 090000, Kazakhstan.
Life (Basel). 2024 Sep 15;14(9):1167. doi: 10.3390/life14091167.
This study evaluates the utilization of in vitro embryo production (IVEP) technology for the conservation and breeding of the Auliekol cattle breed, a primary beef breed in Kazakhstan facing population decline due to the cessation of breeding programs and the incursion of transboundary diseases. We assessed the effect of consecutive ovum pick-up (OPU) procedures on oocyte yield and embryo production in Auliekol and Aberdeen Angus cows. A total of 2232 and 3659 oocytes were aspirated from Auliekol and Aberdeen Angus donors, respectively, with significantly higher yields and embryo production observed in Aberdeen Angus cows. The application of a meiotic block using Butyrolactone I (BLI) and subsequent in vitro fertilization (IVF) protocols was employed, with embryo development monitored up to the morula/blastocyst stage. Results indicated that Auliekol cows exhibited lower oocyte recovery, cleavage, and blastocyst rates compared to Aberdeen Angus cows, likely due to genetic characteristics. Despite the challenges, IVEP presents a valuable tool for the preservation and future propagation of the Auliekol breed, highlighting the need for further research to enhance reproductive outcomes and conservation strategies.
本研究评估了体外胚胎生产（IVEP）技术在哈萨克斯坦主要肉牛品种奥列科尔牛的保种和育种中的应用。由于育种计划的停止和跨境疾病的侵入，奥列科尔牛种群数量正在下降。我们评估了连续采卵（OPU）程序对奥列科尔牛和阿伯丁安格斯牛的卵母细胞产量和胚胎生产的影响。分别从奥列科尔牛和阿伯丁安格斯牛供体中采集了2232个和3659个卵母细胞，阿伯丁安格斯牛的卵母细胞产量和胚胎生产显著更高。采用了使用丁内酯I（BLI）的减数分裂阻滞和随后的体外受精（IVF）方案，并监测胚胎发育至桑椹胚/囊胚阶段。结果表明，与阿伯丁安格斯牛相比，奥列科尔牛的卵母细胞回收率、分裂率和囊胚率较低，这可能是由于遗传特征所致。尽管存在挑战，但IVEP是奥列科尔牛品种保存和未来繁殖的宝贵工具，突出了进一步研究以提高繁殖结果和保护策略的必要性。
