University Hospital RWTH Aachen, Department of Anaesthesiology, Pauwelsstr. 30, 52074 Aachen, Germany.
Respir Physiol Neurobiol. 2011 May 31;176(3):123-9. doi: 10.1016/j.resp.2011.02.009. Epub 2011 Feb 18.
In vivo fibred confocal laser scanning microscopy allows an evaluation of differences in alveolar mechanics between healthy and acutely injured lungs during mechanical ventilation. The aim of this study was to develop new methods for a quantitative analysis of microscopic images in a murine model of experimental acute lung injury (ALI) and to assess the methods' portability to a large animal model. Differences observed in ALI compared to healthy lungs were: reduction of air-filled areas, increase of heterogeneity and increase of shape irregularity. Three indices were developed: the volume air index (VAI) applies an integral over specific signal intensities, the heterogeneity index (HI) and the Heywood circularity index (CI) comprise variances in size and shape of alveolar structures. The differences between healthy and ALI conditions were found to be significant for all of the used indices (VAI: 0.648 vs. 0.443 (p < 0.05), HI: 0.852 vs. 1.348 (p < 0.001) and CI: 1.56 vs. 1.66 (p < 0.001)). The portability of these algorithms to a porcine model was confirmed reaching similar results (VAI: 0.50 vs. 0.35, p < 0.05; HI: 0.62 vs. 1.83, p < 0.05; CI: 1.56 vs. 1.63, p < 0.001). VAI, HI and CI may help to quantify microscopic images of changes in alveolar structure after experimental ALI.
体内光纤共焦激光扫描显微镜可用于评估机械通气过程中健康肺和急性损伤肺之间肺泡力学的差异。本研究旨在为实验性急性肺损伤（ALI）的小鼠模型中的微观图像开发新的定量分析方法，并评估这些方法在大型动物模型中的可移植性。与健康肺相比，在 ALI 中观察到的差异为：充满空气的区域减少、异质性增加和形状不规则性增加。开发了三个指数：体积空气指数（VAI）对特定信号强度进行积分，异质性指数（HI）和 Heywood 圆形度指数（CI）包含肺泡结构大小和形状的方差。所有使用的指数（VAI：0.648 对 0.443（p<0.05），HI：0.852 对 1.348（p<0.001）和 CI：1.56 对 1.66（p<0.001））均发现健康和 ALI 条件之间存在显著差异。这些算法对猪模型的可移植性得到了证实，结果相似（VAI：0.50 对 0.35，p<0.05；HI：0.62 对 1.83，p<0.05；CI：1.56 对 1.63，p<0.001）。VAI、HI 和 CI 可能有助于量化实验性 ALI 后肺泡结构变化的微观图像。
