Ogawa H
Department of Orthodontics, Osaka Dental University.
Nihon Kyosei Shika Gakkai Zasshi. 1990 Dec;49(6):485-96.
The purpose of this study was to investigate the effects of localized thermal enhancement on new bone formation in rats. Sixty four male Wistar rats of 52-day old were used as the experimental animal. The sagittal suture of the rat was expanded with the force of 75 g by means of an expansion appliance. After the force application for 2 days, the expansion appliance was fixed with a composite resin. The infrared ray was applied to the expanded sagittal suture for 20 minutes a day and the temperature, just above the sagittal suture, was measured with a biothermometer. The local heat was given for 5-days. The experimental animals were divided into 4 groups based on the increase of temperature: Control, 1 degree C, 2.5 degrees C and 4 degrees C groups. The sagittal suture of each rat was examined by both light and fluorescent microscopies. And histochemistry for acid phosphatase and tartrate-resistant acid phosphatase (TRAP) were performed. The results obtained were as follows: 1) In control and 1 degree C groups, little reaction of acid phosphatase and TRAP were observed in the sutural tissue except for the marrow and the neighboring area of the capillary vessels. 2) In 2.5 degrees C and 4 degrees C groups, an increased absorptive area of the bone and an active reaction of acid phosphatase were found in the sutural area. 3) In only 1 degree C group, an increased new bone formation was evident in the sutural area. Results obtained suggested that the degree of new bone formation depended on the temperature of tissues concerned.
本研究的目的是探讨局部热增强对大鼠新骨形成的影响。选用64只52日龄的雄性Wistar大鼠作为实验动物。通过扩张装置以75克的力扩张大鼠的矢状缝。施加力2天后，用复合树脂固定扩张装置。每天对扩张后的矢状缝照射红外线20分钟，并用生物温度计测量矢状缝上方的温度。给予局部热5天。根据温度升高情况将实验动物分为4组：对照组、1℃组、2.5℃组和4℃组。对每只大鼠的矢状缝进行光学显微镜和荧光显微镜检查。并进行酸性磷酸酶和抗酒石酸酸性磷酸酶（TRAP）的组织化学检测。结果如下：1）在对照组和1℃组中，除骨髓和毛细血管附近区域外，在缝合组织中未观察到酸性磷酸酶和TRAP的明显反应。2）在2.5℃组和4℃组中，缝合区域发现骨吸收面积增加和酸性磷酸酶活性反应。3）仅在1℃组中，缝合区域新骨形成增加明显。所得结果表明，新骨形成的程度取决于相关组织的温度。
