Physikalisch-Chemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.
Anal Chem. 2011 Mar 15;83(6):2345-50. doi: 10.1021/ac1032758. Epub 2011 Feb 21.
An inexpensive and easy-to-use immunoassay platform for the sensitive detection of analytes is presented. It comprises single-use polymer test tubes and a compact fluorescence reader. The optics for the capture of supercritical angle fluorescence (SAF) has been built into the tubes allowing for the extremely sensitive readout of solid phase immunoassays in real time and without washing steps. One-step sandwich immunoassays with interleukin 2 (IL-2) were carried out with capture antibodies immobilized in the tubes. At a turn around time of 12 min, the limit of detection for IL-2 was 0.27 pM (4.5 pg/mL) and the linear range covered 3 orders of magnitude. The developed technology is also adaptable to well plates and has great potential of replacing the work-intensive and time-consuming enzyme-linked immunosorbant assay (ELISA).
本文提出了一种廉价、易于使用的免疫分析平台，可用于灵敏地检测分析物。它由一次性使用的聚合物试管和紧凑型荧光读取器组成。用于捕获超临界角荧光（SAF）的光学器件已内置在试管中，可实现固相免疫分析的实时、无洗涤步骤的极其灵敏读出。采用固定在试管中的捕获抗体进行白细胞介素 2（IL-2）的一步夹心免疫分析。在 12 分钟的周转时间内，IL-2 的检测限为 0.27 pM（4.5 pg/mL），线性范围覆盖 3 个数量级。开发的技术也可适用于微孔板，具有替代繁琐耗时的酶联免疫吸附测定（ELISA）的巨大潜力。
