University of Nantes, Faculty of Pharmacy, MMS-EA 2160, F-44000 Nantes, France.
Mar Drugs. 2011 Jan 10;9(1):82-97. doi: 10.3390/md9010082.
In the search for bioactive natural products, our lab screens hydrophobic extracts from marine fungal strains. While hydrophilic active substances were recently identified from marine macro-organisms, there was a lack of reported metabolites in the marine fungi area. As such, we decided to develop a general procedure for screening of hydrophobic metabolites. The aim of this study was to compare different processes of fermentation and extraction, using six representative marine fungal strains, in order to define the optimized method for production. The parameters studied were (a) which polar solvent to select, (b) which fermentation method to choose between solid and liquid cultures, (c) which raw material, the mycelium or its medium, to extract and (d) which extraction process to apply. The biochemical analysis and biological evaluations of obtained extracts led to the conclusion that the culture of marine fungi by agar surface fermentation followed by the separate extraction of the mycelium and its medium by a cryo-crushing and an enzymatic digestion with agarase, respectively, was the best procedure when screening for hydrophilic bioactive metabolites. During this development, several bioactivities were detected, confirming the potential of hydrophilic crude extracts in the search for bioactive natural products.
在寻找具有生物活性的天然产物的过程中，我们的实验室筛选了来自海洋真菌菌株的疏水性提取物。虽然最近已经从海洋大型生物中鉴定出了亲水性活性物质，但海洋真菌领域缺乏报道的代谢物。因此，我们决定开发一种筛选疏水性代谢物的通用程序。本研究的目的是比较使用六种代表性海洋真菌菌株进行发酵和提取的不同过程，以确定生产的最佳方法。研究的参数包括：(a) 选择哪种极性溶剂，(b) 在固体和液体培养之间选择哪种发酵方法，(c) 选择菌丝体还是其培养基作为提取原料，以及 (d) 应用哪种提取方法。对获得的提取物进行生化分析和生物评价的结果表明，当筛选亲水性生物活性代谢物时，琼脂表面发酵培养海洋真菌，然后分别通过冷冻粉碎和琼脂酶酶解来提取菌丝体及其培养基，是最佳的方法。在这个开发过程中，检测到了几种生物活性，证实了亲水性粗提取物在寻找生物活性天然产物方面的潜力。
