Syngeneic murine metastasis models : b16 melanoma.

Animal studies are costly, time consuming, and subject to several restrictive regulations. However, the metastatic process is one of the research areas in which the in vivo studies remain most relevant (1). In fact, the in vitro studies are not fully predictive of the metastatic behavior of a tumor cell, probably because in vitro, it is difficult to reproduce the complexity of the metastatic process in itself and the interaction with the host.