Department of Tumor Biology, Third Faculty of Medicine Charles University, Ruska 87, 100 34 Prague, Czech Republic.
Anticancer Res. 2010 Dec;30(12):4799-803.
We report a syngeneic model of spontaneous metastatic B16-F10 mouse melanoma in C57/BL6 mice with a very high metastatic frequency that mimics clinical metastatic melanoma. The B16 melanoma cells were injected between the skin and cartilage on the dorsal side of the ear. The model generated lymphatic and visceral metastases in all of the tested animals. In mice with large primary tumors, tumor weight correlated with the tumor growth time and also with the number of metastases in lymph nodes and organs. The dorsal ear space between the skin and cartilage enables both lymphatic and hematogenous metastatic spread. The model should be useful to study the mechanism of melanoma metastasis and to develop therapy for this currently untreatable disease.
我们报告了一个自发转移性 B16-F10 小鼠黑色素瘤的同基因模型，该模型在 C57/BL6 小鼠中具有非常高的转移频率，模拟了临床转移性黑色素瘤。B16 黑色素瘤细胞被注射到耳朵背面的皮肤和软骨之间。该模型在所有测试的动物中均产生了淋巴和内脏转移。在原发性肿瘤较大的小鼠中，肿瘤重量与肿瘤生长时间以及淋巴结和器官转移的数量相关。皮肤和软骨之间的耳朵背面空间允许淋巴和血行转移扩散。该模型应该有助于研究黑色素瘤转移的机制，并开发针对这种目前无法治愈的疾病的治疗方法。
