Klass D J
J Appl Physiol Respir Environ Exerc Physiol. 1978 Oct;45(4):487-94. doi: 10.1152/jappl.1978.45.4.487.
In vivo levels of guanosine 3',5'-cyclic monophosphate (cGMP) in rat lung were measured by radioimmunoassay. The basal levels of this agent were found to change depending on the method of killing or anesthesia used. Lowest values were found after microwave irradiation to the head (10.2 fmol/mg wet wt). Values after pentobarbitol anesthesia, with or without atropine, were significantly higher (19-24 fmol/mg). In animals anesthetized with pentobarbital and ventilated at pressures of 7 cmH2O with no end-expiratory pressure, lung cGMP was 42-49 fmol/mg. Ventilation at higher pressures resulted in much higher levels of cGMP (20 cmH2O, 105 fmol/mg; 30 cmH2O, 233 fmol/mg). The response of cGMP to ventilation was not blocked by pretreatment of the animals with atropine. High-pressure ventilation was without effect on lung adenosine 3',5'-cyclic monophosphate.
采用放射免疫分析法测定大鼠肺中鸟苷 3',5'-环磷酸（cGMP）的体内水平。发现该物质的基础水平会因处死方法或所用麻醉方式的不同而改变。头部经微波照射后的值最低（10.2飞摩尔/毫克湿重）。戊巴比妥麻醉后的值，无论有无阿托品，都显著更高（19 - 24飞摩尔/毫克）。在戊巴比妥麻醉且以7厘米水柱压力通气且无呼气末正压的动物中，肺cGMP为42 - 49飞摩尔/毫克。更高压力的通气导致cGMP水平大幅升高（20厘米水柱，105飞摩尔/毫克；30厘米水柱，233飞摩尔/毫克）。动物预先用阿托品处理并未阻断cGMP对通气的反应。高压通气对肺腺苷 3',5'-环磷酸无影响。
