Klass D J
Arch Environ Health. 1980 Nov-Dec;35(6):347-50. doi: 10.1080/00039896.1980.10667518.
The enzyme guanylate cyclase is stimulated to produce cycle guanosine 3',5'-monophosphate (GMP) when lung tissue is exposed to cigarette smoke in vitro. These experiments tested whether in vivo exposure in rats to cigarette smoke produces a similar response. Adult rats were anaesthetized with pentobarbital and ventilation with mixtures of air and cigarette smoke at 10 cm H2O inspiratory pressure was achieved after a tracheotomy was performed. Lung tissue samples were taken at intervals during 20 min exposure period and analyzed for levels of cyclic adenosine 3', 5'-monophosphate (AMP) and cycle GMP. Blood carboxyhemoglobin (COHb) levels at 5 min and 15 min of exposure showed high, but sublethal levels of COHb. lung tissue cAMP was unchanged with this exposure, but cGMP levels rose dramatically. Rat lungs showed no changes related to ventilation under similar conditions in the absence of smoke. This observed response of cGMP to cigarette smoke may represent an important pulmonary defense mechanism.
当肺组织在体外暴露于香烟烟雾时，鸟苷酸环化酶被刺激产生环鸟苷3',5'-单磷酸（GMP）。这些实验测试了大鼠在体内暴露于香烟烟雾是否会产生类似的反应。成年大鼠用戊巴比妥麻醉，在进行气管切开术后，以10 cm H₂O的吸气压力用空气和香烟烟雾的混合物进行通气。在20分钟的暴露期内定期采集肺组织样本，并分析环腺苷3',5'-单磷酸（AMP）和环GMP的水平。暴露5分钟和15分钟时的血液碳氧血红蛋白（COHb）水平显示出较高但未致死的COHb水平。这种暴露下肺组织cAMP没有变化，但cGMP水平急剧上升。在没有烟雾的类似条件下，大鼠肺在通气方面没有显示出变化。观察到的cGMP对香烟烟雾的反应可能代表一种重要的肺部防御机制。
