Zhang Chi, Wang Xiao-Xiao, Wang Lu, Xiang Ying, Wei Qing, Wang Wan-Yu, Xiong Yu-Liang, Chen Shi, Chen Gang
Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Key Laboratory of Organ Transplantation, Ministry of Education,Wuhan 430030, China.
Dongwuxue Yanjiu. 2011 Feb;32(1):56-61. doi: 10.3724/SP.J.1141.2011.01056.
Similar to humans, most of non-human primates also contain the ABO blood group system. In this study, we sought to evaluate the ABO antibody (Ab) levels in monkeys using a modified flow cytometry method (FCM). The standard commercial human A or B-red blood cells (RBCs) were used as target cells. The binding of target cells and anti-A or B blood group Ab in sera of rhesus or cynomolgus monkeys was detected by flow cytometry after adding secondary specific fluorescence-labeled anti-human IgG or IgM Ab. Human healthy blood donor sera were used as controls. The results revealed that, using clear monkey sera, which were pre-absorbed on normal human type O RBCs to remove non-specific anti-human Abs, the modified FCM gave an accurate detection of ABO Ab levels in monkeys. When compared with the results of human sera, the distribution of ABO Ab levels in monkey sera were significantly lower (P<0.05). We concluded that the modified FCM could be used for the detection of monkey ABO Ab levels. The technique and data will be very valuable for the future establishment of ABO-incompatible organ transplant models in non-human primates, which could improve clinical applications.
与人类相似，大多数非人灵长类动物也拥有ABO血型系统。在本研究中，我们试图使用改良的流式细胞术（FCM）评估猴子体内的ABO抗体（Ab）水平。使用标准的商业化人A或B型红细胞（RBC）作为靶细胞。在加入二级特异性荧光标记的抗人IgG或IgM抗体后，通过流式细胞术检测恒河猴或食蟹猴血清中靶细胞与抗A或B血型Ab的结合情况。使用人类健康献血者血清作为对照。结果显示，使用在正常人O型RBC上预先吸附以去除非特异性抗人抗体的清亮猴血清，改良的FCM能够准确检测猴子体内的ABO Ab水平。与人类血清结果相比，猴血清中ABO Ab水平的分布显著更低（P<0.05）。我们得出结论，改良的FCM可用于检测猴子的ABO Ab水平。该技术和数据对于未来在非人灵长类动物中建立ABO不相容器官移植模型将非常有价值，这可能会改善临床应用。
