Oldt Robert F, Kanthaswamy Sreetharan, Montes Mae, Schumann Laura, Grijalva Jose, Bunlungsup Srichan, Houghton Paul, Smith David Glenn, Malaivijitnond Suchinda
School of Mathematics and Natural Sciences, Arizona State University (ASU) at the West Campus, Glendale, Arizona.
Evolutionary Biology Graduate Program, School of Life Sciences, ASU, Tempe, Arizona.
Int J Immunogenet. 2019 Feb;46(1):38-48. doi: 10.1111/iji.12405. Epub 2018 Nov 2.
Knowledge of the macaque ABO blood group system has been critical in the development of nonhuman primates (NHPs) as a translational model. Serving not only as a useful homologue of the disease-linked ABO system in humans, macaque ABO blood groups must be typed in colonies prior to performing experimental procedures requiring blood transfusion or transplantation. While the rates of blood type incompatibility and the distributions of A, B and AB blood groups are known in large samples of rhesus (Macaca mulatta) and cynomolgus (M. fascicularis) macaques, there is a dearth of blood type data from macaque populations occupying the rhesus-cynomolgus hybrid zone in Southeast Asia. Using molecular phenotyping, we profiled ABO blood group distributions of 232 macaques from 10 populations in the hybrid zone and compared them to pure blood populations of the two species. We found that while these distributions are significantly different in most populations, there was a lack of differentiation between the hybrid and cynomolgus macaques as well as between the Thai and neighbouring populations. This supports a more expansive model of hybridization between rhesus and cynomolgus macaques than often proposed and highlights the increased need for consideration of population genetic structure in biomedical studies that employ macaques as animal models. Additionally, we report an enrichment of indeterminate blood types in the hybrid populations.
猕猴ABO血型系统的知识对于将非人灵长类动物（NHPs）开发为转化模型至关重要。猕猴ABO血型不仅是人类疾病相关ABO系统的有用同源物，而且在进行需要输血或移植的实验程序之前，必须在群体中对猕猴ABO血型进行分型。虽然在恒河猴（Macaca mulatta）和食蟹猴（M. fascicularis）的大量样本中已知血型不相容率以及A、B和AB血型的分布情况，但东南亚恒河猴 - 食蟹猴杂交区域的猕猴种群的血型数据却很匮乏。我们使用分子表型分析方法，对杂交区域10个种群的232只猕猴的ABO血型分布进行了分析，并将其与这两个物种的纯种群体进行了比较。我们发现，虽然大多数种群的这些分布存在显著差异，但杂交猕猴与食蟹猴之间以及泰国猕猴与邻近种群之间缺乏分化。这支持了一种比通常提出的更为广泛的恒河猴和食蟹猴杂交模型，并强调在将猕猴用作动物模型的生物医学研究中，更需要考虑种群遗传结构。此外，我们报告了杂交种群中不确定血型的富集情况。
