Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan.
J Am Chem Soc. 2011 Mar 16;133(10):3246-8. doi: 10.1021/ja1096122. Epub 2011 Feb 22.
Infectious hepatitis B virus (HBV), namely Dane particles (DPs), consists of a core nucleocapsid including genome DNA covered with an envelope of hepatitis B surface antigen (HBsAg). We report the synthesis, structure, and HBV-trapping capability of multilayered protein nanotubes having an anti-HBsAg antibody (HBsAb) layer as an internal wall. The nanotubes were prepared using an alternating layer-by-layer assembly of human serum albumin (HSA) and oppositely charged poly-L-arginine (PLA) into a nanoporous polycarbonate (PC) membrane (pore size, 400 nm), followed by depositions of poly-L-glutamic acid (PLG) and HBsAb. Subsequent dissolution of the PC template yielded (PLA/HSA)(2)PLA/PLG/HBsAb nanotubes (AbNTs). The SEM measurements revealed the formation of uniform hollow cylinders with a 414 ± 16 nm outer diameter and 59 ± 4 nm wall thickness. In an aqueous medium, the swelled nanotubes captured noninfectious spherical small particles of HBsAg (SPs); the binding constant was 3.5 × 10(7) M(-1). Surprisingly, the amount of genome DNA in the HBV solution (HBsAg-positive plasma or DP-rich solution) decreased dramatically after incubation with the AbNTs (-3.9 log order), which implies that the infectious DPs were completely entrapped into the one-dimensional pore space of the AbNTs.
传染性乙型肝炎病毒（HBV），即 Dane 颗粒（DPs），由包含基因组 DNA 的核心核衣壳组成，被乙型肝炎表面抗原（HBsAg）的包膜覆盖。我们报告了具有抗 HBsAg 抗体（HBsAb）层作为内壁的多层蛋白质纳米管的合成、结构和 HBV 捕获能力。纳米管是通过交替层叠人血清白蛋白（HSA）和带相反电荷的聚-L-精氨酸（PLA）到纳米多孔聚碳酸酯（PC）膜（孔径，400nm）中制备的，然后沉积聚-L-谷氨酸（PLG）和 HBsAb。随后溶解 PC 模板得到（PLA/HSA）（2）PLA/PLG/ HBsAb 纳米管（AbNTs）。SEM 测量显示形成了具有 414±16nm 外径和 59±4nm 壁厚的均匀空心圆柱体。在水介质中，膨胀的纳米管捕获了非传染性的 HBsAg 球形小颗粒（SPs）；结合常数为 3.5×10(7)M(-1)。令人惊讶的是，HBV 溶液（HBsAg 阳性血浆或 DP 丰富溶液）中的基因组 DNA 量在与 AbNTs 孵育后显著减少（-3.9 对数级），这意味着传染性 DP 完全被捕获到 AbNTs 的一维孔空间中。
