Steiert J G, Urbanowski M L, Stauffer L T, Plamann M D, Stauffer G V
Department of Microbiology, University of Iowa, Iowa City 52242.
DNA Seq. 1990;1(2):107-13. doi: 10.3109/10425179009016038.
The DNA sequence of the Salmonella typhimurium glyA gene has been determined. The polypeptide deduced from the DNA sequence contains 417 amino acids and has a calculated molecular weight of 45428 daltons. S1 nuclease mapping experiments located the transcription start point and possible transcription termination region. The nucleotide and amino acid sequences for the S. typhimurium and Escherichia coli glyA genes were compared. The nucleotide sequences show 89% identity, and the amino acid sequences show 93% identity. In S. typhimurium there is an absence of REP sequences between the translation termination site and the proposed transcription termination site that are present in the E. coli sequence. A conserved sequence is found in both organisms extending from 79 to 117 bp upstream of the consensus -35 sequences of the glyA promoters. This conserved sequence shows homology to a sequence preceding the S. typhimurium metE gene determined to bind the MetR regulatory protein.
鼠伤寒沙门氏菌glyA基因的DNA序列已被测定。从该DNA序列推导的多肽含有417个氨基酸，计算分子量为45428道尔顿。S1核酸酶图谱实验确定了转录起始点和可能的转录终止区域。对鼠伤寒沙门氏菌和大肠杆菌glyA基因的核苷酸和氨基酸序列进行了比较。核苷酸序列显示89%的同一性，氨基酸序列显示93%的同一性。在鼠伤寒沙门氏菌中，翻译终止位点与提议的转录终止位点之间不存在大肠杆菌序列中存在的REP序列。在这两种生物体中，在glyA启动子共有-35序列上游79至117bp处发现了一个保守序列。该保守序列与已确定与MetR调节蛋白结合的鼠伤寒沙门氏菌metE基因之前的一个序列具有同源性。
