Park Y M, Stauffer G V
Department of Microbiology, University of Iowa, Iowa City 52242.
Mol Gen Genet. 1989 Mar;216(1):164-9. doi: 10.1007/BF00332246.
The DNA sequence of the Salmonella typhimurium metC gene and its flanking regions was determined. The metC gene contains an open reading frame of 1185 nucleotides encoding a polypeptide of 395 amino acids with a predicted molecular weight of 42,874 daltons. S1 nuclease mapping experiments located the transcription start site of the metC gene. The nucleotide sequence and the deduced amino acid sequence for the metC genes of S. typhimurium and Escherichia coli were compared. Although there are 279 nucleotide replacements, most do not change the amino acid sequence. Nucleotide sequence analysis of the flanking regions of the S. typhimurium metC gene shows that there is an open reading frame upstream and an open reading frame downstream of the gene. The existence of the divergently transcribed upstream open reading frame (designated ORF1) was confirmed by the construction of an ORF1-lacZ fusion. The transcription start site of ORF1 was determined by S1 nuclease mapping.
测定了鼠伤寒沙门氏菌metC基因及其侧翼区域的DNA序列。metC基因包含一个1185个核苷酸的开放阅读框，编码一个由395个氨基酸组成的多肽，预测分子量为42,874道尔顿。S1核酸酶图谱实验确定了metC基因的转录起始位点。比较了鼠伤寒沙门氏菌和大肠杆菌metC基因的核苷酸序列和推导的氨基酸序列。虽然有279个核苷酸替换，但大多数并未改变氨基酸序列。对鼠伤寒沙门氏菌metC基因侧翼区域的核苷酸序列分析表明，该基因上游和下游各有一个开放阅读框。通过构建ORF1 - lacZ融合体证实了反向转录的上游开放阅读框（命名为ORF1）的存在。通过S1核酸酶图谱确定了ORF1的转录起始位点。
