McDonald T L, Thiele G M
University of Nebraska Medical Center, Department of Pathology and Microbiology, Omaha 68105-1065.
Diabetes Res. 1990 Apr;13(4):169-76.
Fifty-nine streptozotocin-induced diabetic rats, not treated with insulin clearly demonstrated one of two protein excretion patterns based on urinary albumin and IgG excretion rates. 63% (35/59) of the diabetic rats developed significantly elevated albumin excretion rates (incipient proteinuria) when compared to age-matched control animals. This condition progressed to a highly elevated albuminuria and increased IgG excretion (overt proteinuria). Analysis of IgG subclass content in these diabetic proteinuric rat urines showed that there was a selective excretion of IgG2b into the urine at a time that correlated with the onset of overt proteinuria. This IgG subclass comprised approximately 75% of the total IgG excreted during progressive nephropathy (in some animals, 100%) and was unique as it was not the predominant subclass in the serum of this group of animals. The other 37% of the diabetic rats (22/59) did not develop either incipient or overt diabetic nephropathy. In fact, some animals in this group had statistically less albumin and/or IgG in their urine than control rats. The subclass IgG2c was detected sporadically in the urine of these diabetic non-proteinuric rats throughout the chronological study even though it was the IgG subclass that comprised the lowest serum concentration of all four IgG subclasses. In control rats (total of 19), urinary albumin and low levels of the subclass IgG2a were detected throughout the chronological study; both of which increased with the age of the rat. However, in contrast to diabetic rats, the subclasses IgG1 and IgG2b were not detected in the urine of control rats until late in the chronological study.(ABSTRACT TRUNCATED AT 250 WORDS)
59只链脲佐菌素诱导的糖尿病大鼠，未接受胰岛素治疗，根据尿白蛋白和IgG排泄率，明确表现出两种蛋白尿排泄模式之一。与年龄匹配的对照动物相比，63%（35/59）的糖尿病大鼠出现白蛋白排泄率显著升高（早期蛋白尿）。这种情况进展为高度升高的蛋白尿和IgG排泄增加（显性蛋白尿）。对这些糖尿病蛋白尿大鼠尿液中IgG亚类含量的分析表明，在与显性蛋白尿发作相关的时间，有选择性地将IgG2b排泄到尿液中。这种IgG亚类在进行性肾病期间排泄的总IgG中约占75%（在一些动物中为100%），并且是独特的，因为它不是这群动物血清中的主要亚类。其余37%的糖尿病大鼠（22/59）未发生早期或显性糖尿病肾病。事实上，该组中的一些动物尿液中的白蛋白和/或IgG在统计学上低于对照大鼠。在整个时间进程研究中，这些糖尿病非蛋白尿大鼠的尿液中偶尔检测到IgG2c亚类，尽管它是所有四种IgG亚类中血清浓度最低的IgG亚类。在对照大鼠（共19只）中，在整个时间进程研究中检测到尿白蛋白和低水平的IgG2a亚类；两者都随大鼠年龄增加。然而，与糖尿病大鼠不同，直到时间进程研究后期才在对照大鼠尿液中检测到IgG1和IgG2b亚类。（摘要截断于250字）
