[非小细胞肺癌中线粒体DNA含量的变化]
[Changes on mitochondrial DNA content in non-small cell lung cancer].
作者信息
Wang Hongmei, Dai Jigang
机构信息
Department of Thoracic Surgery, Xinqiao Hospital, Chongqing 400037, China.
出版信息
Zhongguo Fei Ai Za Zhi. 2011 Feb;14(2):141-5. doi: 10.3779/j.issn.1009-3419.2011.02.07.
BACKGROUND AND OBJECTIVE
It has been proven that the mitochondrial DNA (mtDNA) mutations and content change were associated with increasing risk of tumorigenesis. MtDNA content is significantly reduced in most substantive tumors. The aim of this study is to demonstrate whether mtDNA content is positively associated with non-small cell lung cancer (NSCLC) risk.
METHODS
MtDNA content in 37 matched lung carcinoma and histologically adjacent normal lung tissue samples from patients were analyzed by fluorogenic 5-nuclease real-time PCR techniques.
RESULTS
The mean copy number of mtDNA in lung carcinoma tissue samples was statistically lower than that in adjacent histologically normal lung tissue samples (P < 0.001).
CONCLUSIONS
The change of mtDNA content may play an important role in NSCLC.
背景与目的
已证实线粒体DNA（mtDNA）突变及含量变化与肿瘤发生风险增加相关。在大多数实体瘤中，mtDNA含量显著降低。本研究旨在证明mtDNA含量是否与非小细胞肺癌（NSCLC）风险呈正相关。
方法
采用荧光5-核酸酶实时PCR技术分析37例患者的肺癌组织及其组织学相邻正常肺组织样本中的mtDNA含量。
结果
肺癌组织样本中mtDNA的平均拷贝数在统计学上低于相邻组织学正常肺组织样本（P < 0.001）。
结论
mtDNA含量变化可能在NSCLC中起重要作用。
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