Departamento de Engenharia de Materiais, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
IEEE Trans Biomed Eng. 2011 Jun;58(6):1814-9. doi: 10.1109/TBME.2011.2117425. Epub 2011 Feb 22.
Growth of cells in 3-D porous scaffolds has gained importance in the field of tissue engineering. The scaffolds guide cellular growth, synthesize extracellular matrix and other biological molecules, and make the formation of tissues and functional organs easier. The aim of this study is to use α-tricalcium phosphate cement in order to obtain new types of scaffolds with the aid of paraffin spheres as pore generators. The porosity of the scaffolds produced with paraffin spheres was analyzed and compared to the literature, and the study of scaffold permeability using the Forchheimer equation allowed the analysis of pore interconnectivity. In vitro tests showed the behavior of scaffolds in solutions of simulated body fluid, and viability and cell proliferation were also evaluated. The results show the potential use of the materials developed for scaffolds for use in tissue engineering applications.
三维多孔支架中的细胞生长在组织工程领域受到重视。支架引导细胞生长、合成细胞外基质和其他生物分子，使组织和功能性器官的形成变得更容易。本研究的目的是使用α-磷酸三钙水泥，借助石蜡球作为孔生成剂来获得新型支架。分析了使用石蜡球生产的支架的孔隙率，并与文献进行了比较，使用 Forchheimer 方程研究支架的渗透性可以分析孔的连通性。体外测试显示了支架在模拟体液溶液中的行为，还评估了支架的生存能力和细胞增殖能力。结果表明，所开发的材料具有用于组织工程应用的支架的潜力。
