Schumm D E, Webb T E
J Biol Chem. 1978 Dec 10;253(23):8513-7.
The addition of physiological concentrations of either cAMP or cGMP stimulated the release of RNA from isolated prelabeled rat liver nuclei to a fortified cytosol in a cell-free system. The released RNA was shown to be primarily mRNA by its binding to oligo(dT)-cellulose and its sedimentation profile. Treatment of rats with cAMP or cGMP 30 min prior to the preparation of cyclic nucleotides on the cell-free system. Cyclic nucleotides stimulation of RNA release occurred in systems prepared from resting rat liver, Novikoff hepatoma, and Morris hepatoma 5123D, but not the 18-h regenerating liver. The response of the cell-free system to added cyclic nucleotides reflected the in vivo concentration of these substances in the tissues from which the system was prepared. Those with high in vivo levels were not stimulated while those with lower levels did respond to added cyclic nucleotides. Neither cAMP nor cGMP had an appreciable effect on rRNA release.
在无细胞系统中，添加生理浓度的环磷酸腺苷（cAMP）或环磷酸鸟苷（cGMP）可刺激预先标记的离体大鼠肝细胞核中的RNA释放到强化细胞溶胶中。通过其与寡聚（dT）-纤维素的结合及其沉降图谱表明，释放的RNA主要是信使核糖核酸（mRNA）。在制备无细胞系统之前30分钟，用cAMP或cGMP处理大鼠。环核苷酸刺激RNA释放发生在由静息大鼠肝脏、诺维科夫肝癌和莫里斯肝癌5123D制备的系统中，但在18小时再生肝脏制备的系统中未发生。无细胞系统对添加的环核苷酸的反应反映了制备该系统的组织中这些物质的体内浓度。体内水平高的组织未受到刺激，而水平较低的组织确实对添加的环核苷酸有反应。cAMP和cGMP对核糖体核糖核酸（rRNA）释放均无明显影响。
