Berner A, Børmer O, Marton P F, Nesland J M
Department of Pathology, Norwegian Radium Hospital, Oslo.
Histol Histopathol. 1990 Apr;5(2):199-204.
Three mouse monoclonal antibodies MAB (CEA 12-140-1, -2 and -4) raised against different CEA epitopes were tested in 32 gastric adenocarcinomas (18 intestinal type and 14 diffuse type) and 34 gastric lesions with severe and moderate dysplasia. The MAB stained 13, 11 and 13 out of the 14 diffuse carcinomas and 11, 13 and 13 out of the 18 intestinal carcinomas. The dysplastic lesions were positive in 9, 9 and 6 out of 34 cases. Less than half of the cases with metaplastic epithelium adjacent to the carcinomas were also positive for MAB. All MAB showed the same pattern of reactivity without cross-reactivity. Their cumulative staining rate corresponded closely to that of polyclonal CEA antiserum, but the MAB stained more cells. The reactivity was confined to intracytoplasmic vacuoles in diffuse carcinomas and appeared diffusely in the cytoplasm or limited to the cell membrane in intestinal type of carcinomas. Our findings do not indicate CEA to be a reliable marker for malignant transformation in gastric mucosa.
针对不同癌胚抗原（CEA）表位产生的三种小鼠单克隆抗体MAB（CEA 12 - 140 - 1、- 2和- 4），在32例胃腺癌（18例肠型和14例弥漫型）以及34例伴有重度和中度发育异常的胃部病变中进行了检测。这三种单克隆抗体在14例弥漫型癌中分别染色了13例、11例和13例，在18例肠型癌中分别染色了11例、13例和13例。在34例发育异常病变中，分别有9例、9例和6例呈阳性。癌旁化生上皮病例中，不到一半的病例对单克隆抗体也呈阳性。所有单克隆抗体显示出相同的反应模式，无交叉反应。它们的累积染色率与多克隆CEA抗血清的累积染色率密切对应，但单克隆抗体染色的细胞更多。在弥漫型癌中，反应局限于胞浆内空泡，在肠型癌中则弥漫于细胞质或局限于细胞膜。我们的研究结果表明，CEA并非胃黏膜恶性转化的可靠标志物。
