Stramignoni D, Coda R
Cancer Detect Prev. 1985;8(1-2):193-206.
Two monoclonal antibodies antitumor-associated antigens, B1.1 and B72.3, have been used with the immunoperoxidase technique on tissue sections to study gastric carcinomas, dysplasia, intestinal metaplasia, and adenomas. B1.1 reacts with carcinoembryonic antigen (CEA); B72.3 reacts with a 220-400 kd glycoprotein present in colon, breast, and other carcinomas. CEA was found in 89% (34 of 38) and B72.3 antigen in 92% (35 of 38) of carcinomas. In half of these more than 50% of tumor cells were positive. The normal epithelium was usually negative or sporadically positive in a few cells. In dysplastic areas and adenomas the number of cells that were positive for the two antigens was greater than in normal epithelium and smaller than in carcinomas. In intestinal metaplasia B72.3 antigen was almost always present, whereas CEA was sometimes undetectable. Both the antigens proved to be good markers of neoplastic versus normal cells. The presence of B72.3 antigen in addition to CEA in dysplasia, adenomas, and intestinal metaplasia adds further evidence of their close relationship with gastric cancer.
两种抗肿瘤相关抗原的单克隆抗体B1.1和B72.3已与免疫过氧化物酶技术一起用于组织切片，以研究胃癌、发育异常、肠化生和腺瘤。B1.1与癌胚抗原（CEA）反应；B72.3与存在于结肠癌、乳腺癌和其他癌中的一种220 - 400kd糖蛋白反应。在89%（38例中的34例）的癌中发现了CEA，在92%（38例中的35例）的癌中发现了B72.3抗原。在其中一半的病例中，超过50%的肿瘤细胞呈阳性。正常上皮通常为阴性，或少数细胞偶尔呈阳性。在发育异常区域和腺瘤中，两种抗原呈阳性的细胞数量比正常上皮多，比癌少。在肠化生中，B72.3抗原几乎总是存在，而CEA有时检测不到。这两种抗原都被证明是肿瘤细胞与正常细胞的良好标志物。在发育异常、腺瘤和肠化生中，除了CEA外还存在B72.3抗原，进一步证明了它们与胃癌的密切关系。
