Kim Do-Hyun, Song Chul-Gyu, Ilev Ilko K, Kang Jin U
United States Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, Maryland 20993, USA. do‐
Appl Opt. 2011 Feb 20;50(6):970-4. doi: 10.1364/AO.50.000970.
We investigated a high-precision optical method for measuring the thickness of biological samples regardless of their transparency. The method is based on the precise measurement of optical path length difference of the end surfaces of objects, using a dual-arm axial-scanning low-coherence interferometer. This removes any consideration of the shape, thickness, or transparency of testing objects when performing the measurement. Scanning the reference simplifies the measurement setup, resulting in unambiguous measurement. Using a 1310 nm wavelength superluminescent diode, with a 65 nm bandwidth, the measurement accuracy was as high as 11.6 μm. We tested the method by measuring the thickness of both transparent samples and nontransparent soft biological tissues.
我们研究了一种高精度光学方法，用于测量生物样品的厚度，而无需考虑其透明度。该方法基于使用双臂轴向扫描低相干干涉仪精确测量物体端面的光程长度差。这使得在进行测量时无需考虑测试物体的形状、厚度或透明度。扫描参考臂简化了测量设置，从而实现明确无误的测量。使用波长为1310 nm、带宽为65 nm的超发光二极管，测量精度高达11.6 μm。我们通过测量透明样品和不透明软生物组织的厚度对该方法进行了测试。
