Bordi Licia, Meschi Silvia, Selleri Marina, Lalle Eleonora, Castilletti Concetta, Carletti Fabrizio, Chiappini Roberta, Di Caro Antonino, Capobianchi Maria Rosaria
Laboratory of Virology, National Institute for Infectious Diseases L. Spallanzani, Rome, Italy.
New Microbiol. 2011 Jan;34(1):87-91. Epub 2011 Jan 15.
Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus responsible for the first autochthonous Italian outbreak in 2007. A226V mutation in E1 has been associated with enhanced replication in A. albopictus vector. Possible involvement of this mutation in enhanced infection capability in primate cells and sensitivity to exogenous interferon (IFN)-a was investigated. No significant differences were observed between the two isolates in terms of replication kinetic, virus yield and cytopathic effect (CPE). Interestingly, the A226V-carrying strain was more susceptible to the antiviral action of recombinant IFN-a. The interplay between A226V mutation and innate defence mechanisms needs further investigation.
基孔肯雅病毒（CHIKV）是一种由蚊子传播的甲病毒，它导致了2007年意大利首次本土疫情爆发。E1基因中的A226V突变与在白纹伊蚊载体中的复制增强有关。研究了该突变在灵长类细胞中增强感染能力以及对外源干扰素（IFN）-α敏感性方面的可能作用。在复制动力学、病毒产量和细胞病变效应（CPE）方面，两种分离株之间未观察到显著差异。有趣的是，携带A226V的毒株对重组IFN-α的抗病毒作用更敏感。A226V突变与先天防御机制之间的相互作用需要进一步研究。
