Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou, China.
Theor Appl Genet. 2011 May;122(8):1497-502. doi: 10.1007/s00122-011-1549-0. Epub 2011 Feb 23.
Fusarium head blight (FHB) of wheat causes not only significant reduction in grain yield and end-use quality, but also the contamination of the grain with mycotoxins that are detrimental to human and animal health after consumption of infected grain. Growing resistant varieties is an effective approach to minimize the FHB damage. The Chinese wheat landrace Haiyanzhong (HYZ) shows a high level of resistance to FHB. To identify quantitative trait loci (QTL) that contribute to FHB resistance in HYZ, 136 recombinant inbred lines (RIL) were developed from a cross of HYZ and Wheaton, a hard spring wheat cultivar from the USA. The RIL and their parents were evaluated for percentage of scabbed spikelets (PSS) in both greenhouse and field environments. Five QTL were detected for FHB resistance in HYZ with one major QTL on 7DL. The 7DL QTL peaked at SSR marker Xwmc121, which is flanked by the SSR markers Xcfd46 and Xwmc702. This QTL explained 20.4-22.6% of the phenotypic variance in individual greenhouse experiments and 15.9% in a field experiment. Four other minor QTL on 6BS (two QTL), 5AS and 1AS each explained less than 10% of the phenotypic variance in individual experiments. HYZ carried the favorable alleles associated with FHB resistance at the QTL on 7DL, 6BS and 5AS, and the unfavorable allele at the QTL on 1AS. The major QTL on 7D can be used to improve the FHB resistance in wheat breeding programs and add diversity to the FHB resistance gene pool.
小麦镰孢穗腐病（FHB）不仅导致籽粒产量和食用品质显著降低，而且还导致受污染谷物中的真菌毒素污染，这些毒素在食用受感染的谷物后对人类和动物健康有害。培育抗性品种是减轻 FHB 损害的有效方法。中国小麦地方品种海宴中（HYZ）对 FHB 表现出高水平的抗性。为了鉴定与 HYZ 的 FHB 抗性有关的数量性状位点（QTL），从 HYZ 和美国硬春小麦品种惠顿的杂交后代中开发了 136 个重组自交系（RIL）。在温室和田间环境中，对 RIL 及其亲本进行了病穗率（PSS）的评估。在 HYZ 中检测到 5 个与 FHB 抗性相关的 QTL，其中一个主要 QTL 位于 7DL 上。7DL QTL 在 SSR 标记 Xwmc121 处达到峰值，该标记由 SSR 标记 Xcfd46 和 Xwmc702 侧翼。该 QTL 解释了个体温室试验中 20.4-22.6%的表型方差和田间试验中 15.9%的表型方差。在 6BS（两个 QTL）、5AS 和 1AS 上还检测到另外 4 个较小的 QTL，每个 QTL 在个体试验中解释的表型方差不到 10%。HYZ 在 7DL、6BS 和 5AS 上的 QTL 上携带与 FHB 抗性相关的有利等位基因，而在 1AS 上的 QTL 上携带不利等位基因。7D 上的主要 QTL 可用于提高小麦育种计划中的 FHB 抗性，并为 FHB 抗性基因库增添多样性。
